Nikon Optiphot Quick Reference Manual - Page 7
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Filter Label
Cube Label
FITC
HQ:FITC
TxR
HQ:TXRD
UV
UV-2E/C
Cy5
Cy5
Table 3. Epi-fluorescence filter choices
1/.04 Plan 160/-
2/.05 Plan 160/-
4/.1 EPlan 160/-
10/.40 EPlan 160/-
20/.40 EPlan 160/-
40/.65 EPlan 160/.17
40/.85 Fluor 160/.17*
100/1.30 Oil Fluor 160/.17**
*UV capable, coverslip correction collar
**Variable NA 0.8-1.3
Table 4. Spatial calibration for each objective lens, with the Spot IIe camera.
Objective lens notes.
Other objective lenses are available upon request, such as 60/1.4 oil, 100/1.25 oil.
Lenses designated as "160/-" do not require a coverslip.
Lenses designated as "160/.17" require a coverslip of 170 µm thickness.
July 29, 2016
Digital Microscopy Center, University of Washington
"Color"
green
red
blue
far red
Lens
Part
96170
96172
blue
31023
µm/pixel
pixel/µ
m
8.40
0.12
4.24
0.24
2.12
0.47
0.87
1.15
0.43
2.31
0.21
4.78
0.21
4.78
0.09
11.55
Nikon
C01174
C00178
C81736
1 mm reticle
field
2
820 µm
2
412 µm
2
200 µm
2
200 µm
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