Cole Parmer 1200 Series Gebruikershandleiding - Pagina 14

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flashes. The reading should be 0.000A. If not, repeat step 5.

6. Remove the Cuvette Adapter and insert the Holmium filter into it. Place it in the

Sample Compartment again and close the lid.

7. Record the Absorbance reading on the Digital Display window.

8. Advance the wavelength setting by 1 nm and repeat step 4 to 7.

9. Repeat step 8 until the wavelength setting reaches 370 nm.

10. Look for the maximum absorbance reading obtained, and this should be found within

359~363 nm. The wavelength accuracy of the 1200 is ± 2 nm.

Didymium Filter Method

1. Set the Wavelength to 800 nm.

2. Make sure the cuvette adapter is empty and place it in the Sample Compartment.

Close the lid.

3. Set zero Abs by pressing the ∧ button. Wait a few seconds when the display flashes.

The reading should then be 0.000A. If not, repeat step 3.

4. Remove the cuvette adapter and insert the Didymium filter into it. Place it in the

Sample Compartment again and close the lid.

5. Record the Absorbance reading on the Digital Display window.

6. Advance the wavelength setting by 1nm and repeat step 2 to 5.

7. Repeat step 6 until the wavelength setting reaches 815 nm.

8. Look for the maximum absorbance reading obtained, and this should be found within

805~809 nm. The wavelength accuracy of the 1200 is ± 2 nm.

9. If a "middle" wavelength check is desired, set the wavelength to 522 nm (optional)

10. Make sure the Cuvette Adapter is empty and place it in the Sample Compartment.

Close the lid.

11. Set zero Abs by pressing the ∧ button. Wait a few seconds when the display flashes.

The reading should be 0.000A. If not repeat step 11.

12. Remove the Cuvette Adapter and insert the Didymium filter into it. Place it in the

Sample Compartment and close the lid.

13. Record the absorbance reading on the Digital Display window.

14. Advance the wavelength setting by 1 nm and repeat step 10 to 13.

15. Repeat step 14 until the wavelength setting reaches 536 nm. Again, look for the

maximum absorbance reading. It should be within 527~531 nm.

Absorbance Accuracy Check

Specification: ± 1% at 1A

The absorbance accuracy should be checked against a set of neutral density filters

accurately calibrated to the NIST standards.

An alternative method using potassium dichromate is described below. Due to the many

factors that might affect the results (i.e. temperature, bandpass, weighing and diluting

errors), this method is less accurate and should only be used as a guide.

Reference: Johnson E A

Potassium Dichromate as an absorbance standard