BD FACSAria Procedure voor opstarten, Qc en uitschakelen - Pagina 4
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3. Cleaning Sort Chamber
1
Clean interior chamber as well as plates and sort chamber with ethanol.
1
Make sure all surfaces are dry.
Flow cell. Check for fluid or
salt buildup after removing
closed loop nozzle and
before inserting sort
nozzle.
High voltage deflection
plates. Check prior to sort
and clean with a moistened
kim wipe followed with a
dry one to remove any
liquid.
Waste drawer. To clean, fill
the drawer and the center
catch with dH2O to clear
out any potential blockage.
Tip: While the fluidics startup is
taking place, clean not only the
interior sort chamber but also
all work surfaces on the sorter
and computer table with 70%
ethanol.
1
Sonicate nozzle.
2
In dH2O, NO more than 60 s.
1
Insert the nozzle into the flow cell.
3
Remove the closed loop nozzle from the flow cell, verify the o-ring is in place, gently dry with a kim
wipe and place into nozzle holder. Clean the flow cell with a q-tip if any liquid or buildup is seen.
Inspect the nozzle you will be using for your experiment to verify the o-ring is in place and there is no
clog, and insert it into the flow cell with the o-ring facing up.
Closed loop nozzle – for cleaning and shutdown
Integrated nozzle – for use during setup and sorting