4Deep Desktop Microscope Gebruikershandleiding - Pagina 8

Blader online of download pdf Gebruikershandleiding voor {categorie_naam} 4Deep Desktop Microscope. 4Deep Desktop Microscope 9 pagina's.

The distance between the camera sensor and the point source - Source to Screen Distance

(SSD) of the Desktop microscope is adjustable. Contact 4Deep for the development of custom

conﬁgurations for special applications.

In order to achieve accurate images, inline holography requires that a reasonable amount of

the reference wave (unscattered light) reaches the camera sensor. Too many objects between

the PS and the camera can reduce the amount of reference wave reaching the camera and will

therefore result in poor reconstructions.

For moving objects, keep the exposure time of the camera as short as possible while still

maintaining a visible image. Long exposure times will result in blurry or smeared images. The

exposure can be adjusted in the camera control of the Octopus software.

The PS does not produce a large amount of light; therefore background light from external

sources must be reduced in order to obtain optimal images. Excessive background light can

wash out the images and produce low contrast reconstructions.

If possible, always obtain a background image (a hologram containing only the light from the PS,

without a sample/object causing scatter). A good background image will allow for background

subtraction when reconstructing holograms, producing higher contrast reconstructions while

reducing noise.

9 Care and Maintenance

Your Desktop microscope requires very little maintenance. Keeping the microscope casing

clean and free of lint or dust is recommended. Storing the microscope protected from dirt, dust

and moisture will ensure a long operational life. Take all measures to prevent contact with

the point source, as any dust or liquids on the pinhole may prevent the proper operation

of the microscope.

Problem: Camera is not detected by the software.

Solution: Make sure the power supply connector is properly connected to the microscope.

Make sure the microscope is connected to the Ethernet port on your computer, and that the

power button is on and indicating a current with the light.

Problem: No light from the point source.

Solution: If there is no light coming from the point source and it is properly connected there may

be an alignment problem with the laser, there may be direct damage to the pinhole, or the laser

diode may have burned out. Send the microscope or point source back to the manufacturer for

re-alignment.