Automate ValveLink 8 Gebruikershandleiding - Pagina 6

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Standard Tefl on Perfusion System
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*Green clamps
close to mani-
fold
vacuum
trap
*Unused lines should be replaced with
plugs on manifold, or tem po rari ly
clamped-off just above man i fold to
avoid back bleeding into empty lines.
Priming one line at a time reduces
bub bles.
Connect your vacuum trap line directly
to the outfl ow of the man i fold for run-
ning cleaning solutions and drying your
lines quickly.
Determine dead volume from the
manifold to sample by measuring
time and volume required to fl ush a
colored liquid with a clear one.
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(without pres sure)
Flow rate adjusted by relative height of
reservoirs and included fl ow regulator.
Always calibrate fl ow rates - regulator
values are relative (see below).
Leave tubing between drip pers and
valves long enough to raise and lower
reservoirs.
1) Assemble ringstand.
2) Attach valve unit.
3) Attach reservoir bracket(s).
4) Insert reservoirs.
5) Insert short pieces of 3/16 i.d. tubing
over tops of drippers, then insert
over reservoir nipples.
6) Attach dripper tubing to upper valve
barb (cut as desired, but see note
above fi rst).
7) Insert green clamps on short pieces of
1/16 tubing.
8) Connect tubing between bottom
valve barbs and manifold.
9) Connect fl ow regulator tubing
to manifold outfl ow and your
per fu sion chamber (downsizing if
necessary).
10) Plug numbered valve cables into
ValveLink ports.
Calibrate fl ow rates by running one line
into a graduated cylinder for a fi xed
pe ri od of time.
Tefl on Micro-manifold Use
Your valves should be equipped or retrofi tted with 1/16" o.d.
outfl ow hose barbs. Micro-manifolds are shipped with a short piece of
1/16" o.d. tubing in each hole (inputs and outfl ow). You can either connect
the valves to manifold inports with a piece of 1/16" i.d. tubing over both
the valve barbs and short pieces of manifold tubing:
Valve with 1/16" o.d. barb
1/16" i.d. tubing over barb
and over manifold short tubing
Manifold with short pieces of 1/16" o.d. tubing in each port
... or, for even less dead volume, use a short piece of 1/16" i.d. tubing
over the valve barb, and piece of smaller 1/16" o.d. tubing inserted inside
the valve tubing and manifold ports (you may need to supply extra small
tubing for this option):
Valve with 1/16" o.d. barb
Short piece of 1/16" i.d. tubing over valve barb
Small diameter 1/16" o.d. tubing inserted inside valve tube
and inserted inside manifold ports
Manifold without connector tubing. 1/16" i.d. holes.
Cutting the tubing at an angle will make insertion easier. Cutting the
tubing square, however, will minimize dead volume when inserted as
far as possible into the manifold – being careful not block at the point of
convergence.
Remember to keep all tubing as short as possible.
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