Olympus CX31-P Gebruiksaanwijzing - Pagina 18

Blader online of download pdf Gebruiksaanwijzing voor {categorie_naam} Olympus CX31-P. Olympus CX31-P 36 pagina's. Polarizing microscope

4-7 Immersion Objective

Fig. 20

Using the Immersion Objective

# Always use immersion oil supplied by Olympus.

Using oil other than designated may degrade the condenser lens

surface.

1. Focus on the specimen using all objectives, starting from the lowest-

power objective to higher-power objective.

2. Before engaging the immersion objective in the light path, place a drop

of immersion oil, provided with the 100x objective set, onto the specimen.

3. Rotate the revolving nosepiece to engage the immersion objective and

turn the fine adjustment knob to bring the specimen into focus.

# Since air bubbles in the oil will affect the image quality, make sure

that the oil is free of bubbles.

To remove bubbles, rotate the revolving nosepiece slightly to move

the objective in the oil by one reciprocation or two.

}With the 1.25 condenser, the rated numerical aperture (NA) is the value

when oil is placed between the slide glass and the top lens of condenser.

If oil is not attached there, the NA is about 0.9.

4. After use, remove oil from the objective front lens by wiping with gauze

slightly moistened with an ether (70%)/alcohol (30%) mixture.

Caution in use of immersion oil

If immersion oil enters your eyes or contacts your skin, immediately take

the following treatment.

Eyes: Rinse with fresh water (for 15 minutes or more).

Skin: Rise with water and soap.

If the appearance of the eyes or skin is altered or pain persists, immediately

see your doctor.

(Fig. 20)