Olympus Fluoview FV1000 Overzicht - Pagina 26

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Main Specifications

Laser Light

Ultraviolet/Visible Light Laser LD lasers: 405 nm: 50 mW, 440 nm: 25 mW, 473 nm: 15 mW, 559 nm: 15 mW, 635 nm, 20 mW

AOTF Laser Combiner

Fiber

Scanning and

Scanner Module

Detection

Detector Module

Filters

Scanning Method

Scanning Modes

Photo Detection Method

Pinhole

Field Number (NA)

Optical Zoom

Z-drive

Transmitted Light

Detector unit

Microscope

Motorized Microscope

Fluorescence Illumination

Unit

System Control PC

Power Supply Unit

Display

Optional Unit

SIM Scanner

TIRFM Unit

4th CH Detector

Fiber Port for Fluorescence

Software

Image Acquisition

Programmable Scan Controller

2D Image Display

3D Visualization and Observation

Image Format

Spectral Unmixing

Image Processing

Image Analysis

Statistical Processing

Optional Software

Objectives for BX2 and IX2

(using U-UCD8A-2, IX2-LWUCDA2 and U-DICTS)

Cover glass

W.D.

Description

thickness

(mm)

UPLSAPO4X

0.16

UPLSAPO10X2

0.40

3.1

UPLSAPO20X

0.75

0.6

UPLSAPO20XO

0.85

0.17

UPLSAPO40X2

0.95

0.18

0.11-0.23

UPLSAPO60XO

1.35

0.15

UPLSAPO60XW

1.20

0.28

0.13-0.21

UPLSAPO100XO

1.40

0.12

PLAPON60XO

1.42

0.15

PLAPON60XOSC

1.40

0.12

UPLFLN40XO

1.30

0.2

APON60XOTIRF

1.49

0.1

0.13-0.19

UAPON100XOTIRF

1.49

0.1

0.13-0.19

UAPON150XOTIRF

1.45

0.08

0.13-0.19

Apo100XOHR

1.65

0.1

Spectral Version

Multi-line Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW), HeNe(G) laser (543 nm, 1 mW)

Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control

Continuously variable (0.1%–100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region

Broadband type (400 nm–650 nm)

Standard 3 laser ports, VIS – UV – IR

Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y)

Motorized optical port for fluorescence illumination and optional module adaptation, Adaptation to microscope fluorescence condenser

Standard 3 confocal Channels (3 photomultiplier detectors)

Additional optional output port light path available for optional units

6 position beamsplitter turrets with CH1 and CH2

CH1 and CH2 equipped with independent grating and slit for fast and

flexible spectral detection

Selectable wavelength bandwidth: 1–100 nm

Wavelength resolution: 2 nm

Wavelength switching speed: 100 nm/msec

CH3 with 6 position barrier filter turret

High performance sputtered filters, dichromatic mirrors and barrier filters

2 galvanometer scanning mirrors

Scanning speed: 512 x 512 (1.1 sec., 1.6 sec., 2.7 sec., 3.3 sec., 3.9 sec., 5.9 sec., 11.3 sec., 27.4 sec., 54.0 sec.)

256 x 256 bidirectional scanning (0.064 sec., 0.129 sec.)

X,Y,T,Z,

Line scanning: Straight line with free orientation, free line, Point scanning

2 detection modes: Analog integration and hybrid photon counting

Single motorized pinhole

pinhole diameter ø50–300 µm (1 µm step)

1x–50x in 0.1x increment

Integrated motorized focus module of the microscope, minimum increment 0.01 µm or 10 nm

Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation to microscope

frame

Inverted IX81, Upright BX61, Upright focusing nosepiece & fixed stage BX61WI

External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit

Motorized switching between LSM light path and fluorescence illumination

PC-AT compatible, OS: Windows XP Professional (English version), Windows Vista (English version), Memory: 2.0 GB or larger, CPU:Core2Duo 3.0 GHz,

Hard disk: 500 GB or larger, Media: DVD Super Multi Drive, FV1000 Special I/F board (built-in PC), Graphic board: conformity with Open GL

Galvo control boards, scanning mirrors and gratings, Real time controller

SXGA 1280X1024, dual 19 inch (or larger) monitors or WQUXGA 2560 x 1600, 29.8 inch monitor

2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port, Fiber introduction of near UV diode laser or visible light laser,

Optional: 2nd AOTF laser combiner

Available laser: 405–633 nm. Motorized penetration ratio adjustment. Automatic optical setting for TIRFM objectives

Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path

Output port equipped with FC fiber connector (compatible fiber core 100–125 µm)

Normal scan: 64 x 64, 128 x 128, 256 x 256, 320 x 320, 512 x 512, 640 x 640, 800 x 800, 1024 x 1024, 1600 x 1600, 2048 x 2048, 4096 x 4096

Clip rectangle scan ,Clip ellipse scan ,Polygon clip scan,line scan ,free line scan,Point scan, Real-time image

2-dimension: XY, XZ, XT and X

3-dimension: XYZ, XYT, XY , XZT, XT and XZ

4-dimension: XYZT, XZT and XYT

5 dimension: XYZT

Time Controller function

Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/ ),

LUT: individual color setting, pseudo-color, comment: graphic and text input

Interactive volume rendering: volume rendering display, projection display, animation displayed (save as OIF, AVI or MOV format)

Free orientation of cross section display

3D animation (maximum intensity projection method, SUM method)

3D and 2D sequential operation function

OIB/ OIF image format

8/ 16 bit gray scale/index color, 24/ 32/ 48 bit color,

JPEG/ BMP/ TIFF/ AVI/ MOV image functions

Olympus multi-tif format

2 Fluorescence spectral unmixing modes (normal and blind mode)

Filter type: Sharpen, Average, DIC Sobel, Median, Shading, Laplacian

Calculations: inter-image, mathematical and logical, DIC background leveling

Fluorescence intensity, area and perimeter measurement, time-lapse measurement

2D data histogram display, colocalization

Review station software, Off-line FLUOVIEW software for date analysis.

Motorized stage control software, Diffusion measurement package, Multi stimulation software, Multi area time-lapse software

Condenser for BX2

Correction

Immersion

U-UCD8A-2

ring

(mm)

optical element

—

0.17

U-DIC10

0.17

U-DIC20

—

Oil

U-DIC20

U-DIC40

0.17

Oil

U-DIC60

Water

U-DIC60

0.17

Oil

U-DIC100

0.17

Oil

U-DIC60

0.17

Oil

U-DIC60

0.17

Oil

U-DIC40

Oil

U-DIC60

Oil

U-DIC100

Oil

U-DIC100

0.15

Oil

U-DIC100

Standard 3 confocal Channels (3 photomultiplier detectors)

Additional optional output port light path available for optional units

6 position beamsplitter turrets with CH1 and CH2

CH1 to CH3 each with 6 position barrier filter turret

(High performance filters)

X,Y,T,Z

Line scanning: Straight line with free orientation, free line, Point scanning

Single motorized pinhole

pinhole diameter ø50–800 µm (1 µm step)

Galvo control boards, scanning mirrors

Objectives for fixed stage upright microscope

(using WI-UCD, WI-DICTHRA2)

Objectives

Condenser for IX2

U-DICTS

IX2-LWUCDA2

position

optical element

MPLN5X

UMPLFLN10XW

IX2-DIC10

normal

IX2-DIC20

normal

UMPLFLN20XW

IX2-DIC20

normal

LUMPLFLN40XW

IX2-DIC40

normal

IX2-DIC60

BFP1

LUMPLFLN60XW

IX2-DIC60

normal

IX2-DIC100

normal

LUMFLN60XW

IX2-DIC60

BFP1

XLUMPLFLN20XW

IX2-DIC60

BFP1

IX2-DIC40

BFP1

Note: These conditions are not met in confocal microscopy

IX2-DIC60

BFP1

IX2-DIC100

normal

IX2-DIC100

normal

IX2-DIC100

normal

Filter Version

W.D. (mm)

DIC prism

Revolving

nosepiece

WI-SSNP,

0.10

20.00

–

WI-SRE3

WI-SSNP,

0.30

3.50

WI-DIC10HR

WI-SRE3

WI-SSNP,

0.50

3.50

WI-DIC20HR

WI-SRE3

WI-SSNP,

0.80

3.30

WI-DIC40HR

WI-SRE3

WI-SSNP,

1.00

2.00

WI-DIC60HR

WI-SRE3

WI-SSNP,

1.10

1.5

WI-DIC60HR

WI-SRE3

1.00

2.0

WI-DICXLU20HR

WI-SNPXLU2