Olympus Fluoview FV1000 Gebruikershandleiding - Pagina 12

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Olympus Fluoview FV1000 Gebruikershandleiding
System Overview

/ Features of the FV1000

1-2 Features of the FV1000
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I . System Overview
I. 1 - 2
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The photon counting mode is newly provided to improve the sensitivity and S/N and
to enable quantitative optical intensity measurement. Photon counting makes
possible long hours of quantitative observation by completely eliminating analog-
derived drift. The dynamic range in which photon counting is possible is expanded
using a newly designed wideband head amplifier and processing circuitry.
High-speed imaging at 8 frames per sec. is made possible by fast galvano mirror. In
addition, high-speed image acquisition is possible without stopping the Z-series
motors used in the XYZ and XZ observations.
During long hours of time-lapse observation, a stable supply of excitation light is
made possible thanks to the feedback control of the intensity of each laser.
Together with the photon counting function, this function ensures the stability and
quantitative nature of long-hour observations.
Three fluorescence channels, three lasers and AOTF are provided as standard to
meet a large variety of applications.
With a fully-motorized scan unit and motorized microscope, the entire system is
motorized so the scanning conditions including those of the optics can be saved
and reproduced.
When an extension laser irradiation unit is used for photon activation aiming at
causing discoloration, optical simulation or uncaging of the specimen, a system
optimized for cell function analysis experiments can be built.
When the system incorporates the spectral detector unit that is composed of a 2-
channel spectral detector and 1-channel filter, it is possible to set the detection
conditions more flexibly, acquire the fluorescence spectral data and use the
fluorescence isolation function.