Olympus FV3000 Veiligheidshandboek - Pagina 15

Blader online of download pdf Veiligheidshandboek voor {categorie_naam} Olympus FV3000. Olympus FV3000 16 pagina's.
Ook voor Olympus FV3000: Bedieningshandleiding (38 pagina's), Bedieningshandleiding (5 pagina's)

Pagina afdrukken

Stage movement is

not smooth/jerks

around

No signal during

confocal scanning

Signal image not

visible/too dim

even at high laser

power

Control panel

switches off when

Live View is

activated

1. Switch off the stage controller

2. Manually manipulate the stage to each of the 4 corners

3. Spray compressed air to dislodge any dust particles that may be

causing issues.

4. Switch on the stage controller and restart the Fluoview

software

5. Select "Yes" when you see the popup (shown below) asking if

you want to execute the cleaning process for the stage unit.

Use the transmitted detector to check. If the specimen is thick

and non-transparent, the specimen should be removed to first

check for laser pass through to transmitted detector. For easier

verification, standard specimen slides can be used as

confirmation check.

2. Check to see if scanning spot can be spotted on the specimen.

3. If there is no scanning spot:

Check that main laser key is switched on and the

appropriate laser is switched on.

II.

Check the laser rack to check the laser status light.

Blinking light on the diode laser (which indicates laser

starting up) has turned to a solid white or coloured light

(sometimes red light might be indicative of error).

III.

Restart the main controller right at the bottom if none of

the steps taken work, especially if the machine has been

left on by the previous user.

Check: If fluorescence signal is visible on wide-field eyepiece.

Check: PMT settings if fluorescence can be seen on wide-field

Ideal base settings are

• HV: 350-500V for HSD, 600-800V for SD. You can set the HV

higher for quick sample spotting, then when you find the sample

you can tune the HV lower and increase laser power.

• Gain: 1x. Decreasing Gain reduces brightness and increasing

Gain doesn't increase the sensor's sensitivity anyway.

• Offset: 0%. Adjust it only after you find your sample.

• Pinhole: Auto. When set to manual mode it may default to the

smallest size which will make the signal very dark.

This is a feature by Olympus to allow a fully dark room during imaging

that can be switched on/off when needed.

To check status, go to Configuration > Preference tab > Microscope Link

and check Turn the TPC backlight off during scanning and Turn the LED

indicator off during scanning. You can set them to your preferences.

Last updated on Mar 2022