Olympus IX70 Bedieningshandleiding - Pagina 21

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Multi Dimensional Acquisition

MDA main

Be sure to select YOUR directory and a name; images

automatically save there!

Pick dimensions to run (other tabs don't appear unless you check those functions here)

* Camera parameters (gain and offset, yes; NOT exposure) are those from Acquire/acquire dialog EXCEPT use these

buttons to set binning and camera subregions

set #wavelengths desired, then "current

wavelength" to work on (pulldown will list 3

items in this example), THEN define current

identity and exposure for that setting. Repeat for

all illuminations. (each can/should be different)

You can have software autoexpose a wavelength {never, first time, every nth, always}, and you can have it find focus

(defined as highest contrast) similarly as long as you are not also doing a Z series.

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MDA setup Timelapse

(can enter "0" for number of timepoints to run

indefinitely==until stopped manually)

do LIVE, focus to top and bottom, setting

limits with appropriate buttons. Set step size

and/or number of slices to collect. This

routine will NOT import limits from FOCUS

dialog