Olympus IX81-ZDC2 Gebruiksaanwijzing - Pagina 15
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TROUBLESHOOTING GUIDE
Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems
occur, please review the following list and take remedial action as needed.
If you cannot solve the problem after checking the entire list, please contact Olympus for assistance.
Problem
a) Autofocusing is impossible.
b) Autofocusing takes long time or
fails.
c) AF is applied to a position deviated
from the target position.
d) The specimen moves in the X-Y
directions during autofocusing.
Cause
The AF port switching lever is not set to IN.
Bubbles are present in the oil or water. Remove the bubbles.
An objective other than specified is in
use.
The objective is mounted improperly.
The transmitted light bulb generates
infrared light.
A plastic bottom dish is in use.
Electrical noise is interfering with op-
eration.
The glass bottom dish has a large
scratch.
A phase contrast objective is in use.
Immersion oil (or water) of oil- or water-
Immersion objective is dried.
A water-immersion objective is used and
the interface between the water and
glass bottom dish is focused.
AF is set improperly.
Electrical noise is interfering with op-
eration.
There is significant vibration in the in the
installed environment, or an anti-vibration
bench is not used.
The specimen is vibrating.
The offset value is input improperly.
Immersion oil (or water) of oil- or water-
Immersion objective is dried.
A water-immersion objective is used and
the interface between the water and
glass bottom dish is focused.
The specimen is not locked.
Remedy
Set it to IN.
Use another objective or use manual
focusing.
Screw in the objective firmly.
Close the shutter or turn off the light
bulb during AF.
Use a glass bottom dish.
Ground the IX2-UCB. Be also sure to
ground the ancillary equipment.
Use another glass bottom dish or use
manual focusing.
Use the specified objective.
Supply immersion oil (or water).
Set the AF zone so that its center is
located on the interface between the
culture fluid and glass bottom dish and
the AF zone width is ±10 µm.
Set up AF properly.
(For the setting method, refer to the
instruction manual for the application
software.)
Ground the IX2-UCB. Be also sure to
ground the ancillary equipment.
Be sure to use an anti-vibration
bench.
Lock the specimen firmly. Also confirm
that the stage center plate is free of
abnormality.
Enter the correct offset value. (For the
setting method, refer to the instruction
manual for the application software.)
Supply immersion oil (or water).
Set the AF zone so that its center is
located on the interface between the
culture fluid and glass bottom dish and
the AF zone width is ±80 µm.
Lock the specimen by placing a
weight on the glass bottom dish, for
example. Also confirm that the stage
center plate is free of abnormality.
IX81-ZDC2
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