Nikon ECLIPSE 80i Gebruiksaanwijzing - Pagina 39

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Fluorescence photomicrography

20.6

The fluorescence of fluorescent specimens may fade during exposure. To prevent this, do the

following:

(1)

Select a brighter optical combination.

Even if the overall magnification is the same on the monitor, the combination of

objective and camera zoom can result in significant variations in exposure time. We

recommend increasing the magnification with the objective rather than the zoom.

(Generally, the aperture of the objective increases with magnification. The larger the

numerical aperture, the brighter the resulting image.)

(2)

Adjusting the excitation light

Excessively bright excitation light will accelerate specimen fading while making it more

difficult to acquire suitable fluorescent images. Use ND filters to adjust brightness.

(3)

Specimen

Photomicrography of faded specimen sections requires prolonged exposure times and

results in poor color reproduction and low-quality images. Move the specimen to obtain

images from a fresh section of the specimen previously unexposed to excitation light.

Use the differential interference contrast or phase contrast methods to select a

specimen section for photomicrography. For best results, switch to the fluorescent

method to capture images.

(4)

Supersensitive TV camera

Under certain conditions, it may help to place an IR (infrared ray) blocking filter in

front of the supersensitive TV camera sensor. Experiment with the IR blocking filter to

determine its characteristics.

Individual Operations

20 Image Capture