Nikon Eclipse Ti TIFT 1454 Handmatig - Pagina 5

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Metamorph - Taskbar
• On startup your personal taskbar is loaded
• Metamorph functions can be accessed easily from a
Taskbar and an
Acquire window
•
You can restore illumination settings with the
button: "Restore OIC settings"
•
Open the Acquire window with Acquire
•
Access Acquisition modes with: MDA & Stream
• Select preferred illumination settings
o Left side: lasers for camera,
o right side: mercury lamp or transmission to
eyepiece
• Open FRAP or laser console
Metamorph - Acquire window
Sidebar left:
• Exposure time
Minimum interval time at full chip is 90ms, quad chip is 50ms
o
Exposure time can be lower than interval time
o
• Full Chip, Quad Chip, Use Active Region
Use full chip, middle quadrant, or region selected by ROI
o
• Live bin & bin
Combine pixels to increase readout speed
o
Use only for live viewing or real recording
o
Normal state = 1
o
• Start Live/Stop Live
Start/stop live mode
o
Acquire images
• To acquire live mode press "Acquire" button
• Separate images can be recorded as separate images or in stack
Martijn de Gruiter
Manual: TIRF Microscope
Erasmus OIC
Ee-1454
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