Consort E3100 Podręcznik - Strona 12
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Troubleshooting
Troubleshooting
Equipment problem
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Electrophoresis problem
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10
10
Bubbles do not appear on the electrodes:
Ensure that the power supply and the whole electric assembly
is operating properly.
Melted agarose leaks when casting:
Ensure the agarose is not too hot when poured.
Ensure that the sealing surfaces of the running tray and the
gel casting gates are clean.
Ensure that the ends of the running tray are fl at and free of
Ensure that the ends of the running tray are fl at and free of
nicks.
Sample well deformed:
Allow the gel to set for a minimum of 30 minutes.
Leave comb in position until gel returns to room temperature
before removing.
Remove the comb both slowly and at a slight angle to prevent
gel from breaking.
Avoid damaging the well with the pipette when loading the
sample. Aim for the centre of the well and avoid damaging
the bottom of the well with the pipette tip.
Samples leak underneath the gel upon loading:
The bottom of the wells were torn when the comb was re-
moved. To avoid this tearing, carefully wiggle the comb to
free the teeth from the gel.
Distorted sample wells:
Incomplete polymerisation produces poorly defined wells.
De-gas gel solution prior to casting and increase ammonium
persulphate and TEMED concentrations.
Samples do not run straight:
Comb may be warped and should be replaced.
Running tray may be warped and should be replaced. Reduce
the voltage.
Choose a buffer with suitable ionic strength and buffering
capacity.
"Smiling" along one edge of the gel:
Gel was not level when cast or run. Use a gel levelling table
to ensure that the apparatus is level prior to gel casting and
electrophoresis.
E3xxx
E3xxx