Automate ValveLink 8 Podręcznik użytkownika - Strona 5

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Pressure Regulated Perfusion System

AutoMate Scientifi c now offers a Pressurized Perfusion Upgrade

with either four or eight channels for:

• Faster Switching

• Microliter fi ne delivery

• Small diameter pipette delivery

For Single-cell or Whole-cell Superfusion

Multi-barrel Perfusion Pencil

8 micro-bore tubes

into 1 outfl ow

• Single cell and

patch delivery

• Rapid solution

change

• Micro-liter dead

volume

• 250 & 100 micron

removable tips

• Steady fl ow-rate

• Microinjecting

The package can be added to

any new or existing per-

fu sion rig - from AutoMate,

any third-party man u fac -

tur er, or even homemade.

Connect to house air, tank,

or compressor (30 to 100

psi). Precision regulator

delivers zero to 10 psi.

™

Perfusion System

vacuum

trap

*Unused lines should be replaced with

plugs on manifold, or tem po rari ly

clamped-off just above man i fold to

avoid back bleeding into empty lines.

Priming one line at a time reduces

bub bles.

Connect your vacuum trap line directly

to the outfl ow of the man i fold for run-

ning cleaning solutions and drying your

lines quickly.

Determine dead volume from the

manifold to sample by measuring

time and volume required to fl ush a

colored liquid with a clear one.

System Set-up Diagrams

Instructions on this page for Econ-

o my per fu sion systems, Lure-lock

fi ttings, Pres sur ized systems or Pinch

valve systems. (Regular hose barb

Tefl on perfusion systems please see

the next page.)

Lure-lock systems should also see

photo on page 3. Find additional

photos and diagrams of the pressure

system on pages 4 and 8.

Flow rate adjusted by relative height of

reservoirs and included fl ow regulator.

Always calibrate fl ow rates - regulator

values are relative (see below).

Leave tubing between reservoirs and

valves long enough to raise and lower

reservoirs.

1) Assemble ringstand.

2) Attach valve unit.

3) Attach reservoir bracket(s).

4) Insert reservoirs.

5) Attach provided tubing to upper

valve barb (cut as desired, but see

note above fi rst).

6) Connect tubing between bottom

valve barbs and manifold.

7) Connect fl ow regulator tubing

to manifold outfl ow and your

per fu sion chamber (downsizing if

necessary).

8) Plug numbered valve cables into

ValveLink ports.

Calibrate fl ow rates by running one line

into a graduated cylinder for a fi xed

pe ri od of time.