Olympus BHS-312 Instrukcja obsługi - Strona 17

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Olympus BHS-312 Instrukcja obsługi
4. Color and B & W Photomicrography
a) Color photomicrography
*
Voltage setting
........
Within a range as marked with the letters "PHOTO" on
the voltmeter.
©
EUR
ran
vu sara é Insert the LBD-2 filter into the filter mount at the light
exit on the microscope base.
(in case of daylight film)
+
Lightintensity
........
Should
be adjusted by means of the ND filters rather
than by means of voltage change.
The
range
of voltage setting
mentioned
above
indicates a guide line for color photomi-
crography.
If the
photograph,
taken
this way,
however,
gives
bluish
tint, lower the
voltage slightly, and if it gives reddish tint, raise the voltage slightly.
b) Use of contrast filters for B & W photomicrography
©
Green filter
.....
This filter is most recommended, since it aids in obtaining photo-
micrographs of highest resolution and maximum contrast.
Recommended as contrast filter when it is desirable to emphasize
the blue color in the stained portion of a specimen.
©
Cobalt filter
....
Not
generally
recommended
for
photomicrographic
purposes
since it reduces both resolution and contrast.
Use it for specific
applications only when
it is desirable to emphasize yellow in the
stained portion of a specimen.
©
Nofilter
.......
A photograph is taken with less contrast than with the green filter.
© Yellow filter
5. Focusing Procedure
Use the field of view eyepieces for focusing on the film plane.
Each
field of view eyepiece
has a focusing front lens and a reticle with 4 frames, each frame indicating the area covered
by a specific power NFK
photo eyepeice.
(Fig. 22).
The
number
at each
frame
indicates
the
magnification
of
the
photo
eyepiece.
The
image
in the field of view eyepiece and the
image
on
the film
plane are in focus at the
same
time.
Several
type
field of view eye-
pieces
are
available,
according
to
the film
size employed.
Fig. 22
Field of view eyepiece | 35WHK10X | PWHK10X
4X5WHK10X | MHWHK10X
35mm Back | 3% x 4%"
4"x 6"
16mm Bolex
Polaroid
Sheet Film or
camera
Acme camera
Back
Polaroid Film | 120 Roll Film
Holder
Holder
1) Select the field of view eyepiece matching the camera back in use and insert it into the
right eyepiece tube of the trinocular tube, aligning locating groove and
locating pin.
2) While
looking through
the field of view eyepiece, rotate the eyepiece front lens in screw
mount
to focus on the double cross lines in the field.
For sharp focusing with objectives
4X or lower, the focusing magnifier FT is recommended
3) Bring
the
specimen
detail to be photographed
within
the
frame
corresponding
to the
power of the NFK eyepiece in use and focus on the specimen with the microscope fine
adjustment
knobs.
Make sure the light path selector knob on the observation
tube is
either on the white
(V) or yellow-green
(CV)
band.
4) It is recommended
to tighten
the tension adjustment
ring considerably
to prevent the
stage from
dropping during long exposures.
15