Olympus FV3000 Instrukcja obsługi - Strona 32

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（acquiring by multiple channels）

②

⑧

⑤

⑥

Lambda series

①

③

④

⑦

*Before starting the following procedure,

make adjustments for XY imaging.

(refer to page 4-5.)

Changing to Lambda mode

① Select "Lambda" in [Mode] in [PMT

setting] Tool Window.

Select one channel to be used

② Press the

& Detector Select] dialog box appears.

Press the

button to reset the

Assigned Detector.

③ Select the [Channel] tab in [Dye &

Detector Select] dialog box. Select the

detector to be assigned to the

observation channel in [Detector List],

and press

button.

Press the

button to close the [Dye &

Detector Select] dialog box.

④ Tick the checkbox of the wavelength of

the excitation laser to be used.

Setting DM and SDM

⑤ Select [Lightpath] in [Tool Window]

menu. [Lightpath] Tool Window appears.

⑥ Select [LSMScanner] tab in [Lightpath]

Tool Window. Press the DM button to

display the dichroic mirror list. Select

"DM" which reflects the wavelength of

the excitation laser selected in ④.

⑦ Check the light path whether the light

path is set properly.

Setting "Band width" and "Step Size"

⑧ In [PMT Setting] Tool Window, set the

width of the wavelength for which the

photometry is performed per each step

in [Band width], and set the interval

between the wavelength to start

acquisition in the next step in [Step size].

(See page 26 "Standard" for details. )

button. The [Dye