Olympus IX70 Instrukcja obsługi - Strona 7

Przeglądaj online lub pobierz pdf Instrukcja obsługi dla Mikroskop Olympus IX70. Olympus IX70 32 stron. Inverted fluorescent microscope
Również dla Olympus IX70: Instrukcja obsługi (5 strony)

Drukuj stronę

Importantly, there are two filter sliders to attenuate light (ND = neutral density filters) one

on the filter changer just in front of the arc lamp housing, and one in front of the filter changer (Fig. 4).

The filter changer (back) slider imposes an ND2 (100x less light, or 1% illumination), and the scope

(front) slider produces 25% illumination. You should use as little light for live cell imaging as possible

(both sliders pushed in) to forestall photodamage. Most imaging can be done with only the 25% slider

pushed in (well suited for your eyes, too). You can use full light for dim samples, but be aware that you

are probably getting high autofluorescence and probes will bleach quickly. And remember to always

check your controls with the same illumination intensities!

Position

Whl A (F1)

(excitation)

Closed

Open

340

380+nd1.0

440/20

480/40

560/40

640/20

Open

Need special filters? Ask the staff about them... we have more filters than turret positions, so CFP, YFP,

Chameleon FRET, FURA, TexRed and Cy7 cubes are available but not installed.

How-To Do (non-ratio) Fluorescence Image Capture

Much of the configuration of the microscope is manual, but the software will queue you

with pop-up windows to change what needs to be changed by hand. Turn on the computer and logon; start

MetaMorph. MetaMorph (see Figure below) is configured to always start with the 20x objective in place

and cubes set to DIC. The software will pop up a window reminding you to change the objective to the

20x to start. The TASKBAR that pops up contains most of the buttons to select an objective, rotate in

filter cubes, select light paths, operate the camera, etc. If the taskbar does not open, you can find it under

Journal

Taskbars

PDF Created with deskPDF PDF Writer - Trial :: http://www.docudesk.com

Whl B (F2)

following chart reports the contents of the 10 filter slots in each wheel:

(emission)

The Whl-A 340/380 positions are used to conduct calcium imaging

Open

studies with the indicator fura-2. The 440 exciter and Whl-B 485/535

450/65

emission filters are used for real-time FRET probes such as cameleon

485/40

sensors. Both of these methods will require the proper dichroic mirror

535/30

(filter cube) to be placed into the microscope (not routinely installed at

polarizer

this moment). We are swapping them to the #3 filter cube position since

colorRED

few high-speed studies require combinations of fura-2 plus FRET plus

colorGRN

DIC. ColorRED is (600nm LP), colorGRN (500-575 BP), and

colorBLU

colorBLU (500nm SP) filters are intended for (additive) RGB color

630/60

capture, but can be used in custom filter designs as well.

682/22

Load Taskbar. Choose the "main" taskbar. When you choose a function on the

{optional details} For those who want to know the details, the

taskbar, a small window will pop up to

tell/remind you if you need to change

something manually.

1) You should always use the main menu

(top bar) to open the ACQUIRE

menu. This menu gives you access to

camera controls. Other ACQUIRE menu

options include acquisition of multiple

wavelengths, Z series, timelapse, etc. Many

of those menus close after you use them, so

you'll have to go back and re-open them for

more data. If you are collecting multiple

acquire