Olympus IX81 Broszura i specyfikacje - Strona 13

Przeglądaj online lub pobierz pdf Broszura i specyfikacje dla Mikroskop Olympus IX81. Olympus IX81 16 stron. Ix2 series research inverted system microscope

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Spinning Disk Confocal

Obtaining confocal images easily by use of an arc light source.

Disk Scanning Confocal Microscope System

The Olympus Disk Scanning Unit (DSU) offers confocal images

using a white light, arc excitation source and CCD camera. The

heart of the system is a unique slit disk pattern, that offers excellent

light throughput and thinness of optical Sectioning. Compatible with

any IX71 and IX81.

• Compliance with various fluorochromes with different spectral

characteristics.

Since an arc light source is used, the unit can meet different

fluorochrome requirements across a wide wavelength spectrum by

simply switching a standard mirror unit.

• Minimize excitation light damage to the specimen and

maximize emission light throughput.

The excitation light volume is reduced to around 5% as a result of

passing through the disk. So, there is almost no fading of

fluorescence emission from the surface of the focused sample.

• Construction of 3D images.

Brilliant 3D image can be easily captured with excellent optical

sectioning with high precision motorized Z axis of IX81.

• Low and high magnification objective support.

Five DSU disks are available of varying slit spacing and width for the

wide variety of the objectives, included oil or water immersion high

N.A. objectives.

• Easy switching between confocal and reflected light

fluorescence observation .

IN/OUT of the confocal disk to or from the light path can be done

by a hand switch or via software, so it is easy to switch observation

methods between DSU and reflected light fluorescence.

Zebrafish 3-day embryo, ventral view, projection of 62 serial optical sections

Adult brain of Drosophila, reflected light fluorescence image (left) and DSU image (right)

* Not available in some areas

ARC EVA

World's first evanescent illumination system from an arc lamp source.

TIRFM (Total Internal Reflection Fluorescence

Microscopy) system with arc lamp source

Featuring the Olympus-developed total internal reflection

illumination system and slit mechanism to provide evanescent wave

illumination from an arc lamp source. High signal to noise

fluorescence observations with extremely thin optical sectioning can

now be easily performed at the specimen-coverslip interface.

The arc lamp is focused on an off-center slit using a wedge prism

and focused on the outer edge of the back focal plane of the

objective, thus causing the excitation light to exit the objective

beyond the critical angle resulting in Total Internal Reflection.

The wedge prism and slit can be easily removed from the light path

via a slider for wide field fluorescence observation. Through the use

of filters, this system

enables a wider choice of

excitation colors than

current laser base

system.

IX2-ARCEVA

High-precision fluorescence turret /IX2-RFACEVA

Turret includes three, highly precise,

empty fluorescence filter cubes that

permit dichromatic mirror switching

while maintaining excitation light

position on the back focal plane of

the objective. This system makes

multi-color observations easy and

alleviates the additional adjustment

of the excitation source when

switching mirror units. Up to six

mirror units can be installed.

Exclusive

L shape fluorescence

objective

illuminator

High precisiton

fluorescence turret

Off-center slit slider

Wedge prism slider

Excitation filter slider

Fluorescence lamp housing

Main specifications

SYSTEM DIAGRAM

Microscope

Research inverted system

microscope IX71

Fluorescence

Arc illumination total internal reflection

illuminator

fluorescence unit IX2-ARCEVA

IX2-RFAL

(Slit slider, wedge prism slider and

L shape fluorescence

excitation filter slider)

illuminator

L-shape fluorescence illuminator

IX2-RFAL

Mirror unit cassettes

High-precision fluorescence turret

(choose from either

IX2-RFACEVA

fluorescence turret)

(with centering mechanism and

3 vacant mirror units)

Fluorescence turret

APON60XOTIRF

IX2-RFAC

objective

Lamp light source

100 W mercury lamp,

75 W Xenon lamp

Objective

APON60XOTIRF

High

sensitive

N.A. 1.49. W.D. 0.1 mm

camera

Used with normal cover glass and

immersion oil

Stage

Left short handle stage IX-SVL2

Total internal reflection

illumination F.N.

Observation

Recommend high sensitive camera

Application System

Conventional fluorescence observation

TIRFM observation

Microtubule of an NG108-15 cell labeled with Alexa488 through indirect fluorescence antibody test

Kaede-Crk II protein expressed in a HeLa cell

Motorized filter wheel

IX2-ARCEVA

U-LH100HG

Excitation filter slider

100 W mercury

lamp housing

Wedge prism slider

U-LH100HGAPO

100 W mercury apo

lamp housing

Off-center slit slider

IX71

Research

inverted

IX2-RFACEVA

system

microscope

Exclusive

High-precision

vacant mirror unit

fluorescence turret

U-LH75XEAPO

75 W xenon apo

lamp housing

Camera

Mirror unit

IX2-RFAC

adapter

Fluorescence turret

LIFE TIME

BURNER ON

U-RFL-T

Power supply unit

for mercury lamp

LIFE TIME

BURNER ON

U-RX-T

Power supply unit

for xenon lamp

* Not available in some areas