Olympus PROVIS AX70 Przegląd - Strona 11

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Field stop and aperture stop units

for easy attachment of laser and

other special illuminators.

Removable field stop and aperture

stop units facilitate simple attachment

and detachment of laser and

additional illuminators. This versatility

permits application-specific incident

light illumination. In particular, it

enables laser illumination for laser

manipulation and caged compound

release photoactivation.

Twin-lamp housing configuration

with a quick, one-touch illumination

changeover.

Two lamp housings can be mounted

simultaneously onto the AX70 to

significantly expand the microscope's

potential. Infrared IR excitation and

Application example

PAF (Photoactivated Fluorescence)

The PAF technique irradiates caged compound with an ultraviolet ray, instantly transforming

them to original active substances. The induced reaction is then measured and examined.

PAF also allows control of intracellular reaction time and location.

Applications

Examining muscle contraction due to increased intracellular Ca

concentrations.

Study of cytoskeleton assemblies.

Ion channel research.

Study of receptor-bonding of neural transmitters and their functions.

AX70 – the obvious choice

The AX70 incorporates a vertical illuminator with

sideways illumination capability.

The Y-shape also provides an uncluttered work surface for conveniently

positioning an injector or other ancillary equipment near the stand. An

upright microscope is required to observe internal structures of cells

(with samples usually measuring 100 - 200 m thick).

ILLUMINATION

VERSATILITY

Photoactivation of caged fluorescein.

Caged fluorescein labeled tubulin had been introduced in the cell; after

exposure to UV light through a slit a photoactivated zone was generated.

conventional ultraviolet UV excitation

are both required when fluorescence

microscopy moves toward multi-

staining. However, this requires

switching the light source from the

conventional mercury burner to a

halogen source. The twin-lamp

housing configuration on the AX70

makes the switchover between the

mercury burner and halogen source a

simple operation.

Quick excitation light switchover

– a convenient feature for various

photometry and measurement

applications.

Working in combination with the

OSP100-DNA photometry system, the

AX70 easily handles different

measuring methods using several

fluorescent indicators. These range

from 1-wavelength excitation/

1-wavelength photometry 1Ex1Em to

2-wavelength excitation/2-wavelength

photometry 2Ex2Em . Thus, the

AX70/OSP100 combination can

measure fluorescence intensities at a

single wavelength with dual-wavelength

excitation using the fura-2 or BCECF

indicator. These advantages make it

ideal for analyzing intracellular calcium

ion concentrations and pH values.