Nikon LABOPHOT-POL Instrukcja obsługi - Strona 15

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V.PHOTOMICROGRAPHY

Prepare the following equipments in addition to

the LABOPHOT-POL microscope main body.

Nikon Microflex

Trinocular eyepiece tube "TP"

CF PL Projection Lens

1. CF Pl Projection lenses

The combined use of the CF P objectives and

CF PL Projection lenses is essential.

For the same total magnification,

select a com-

bination of the highest possible objective power

and lowest possible projection

lens power to

achieve

the

utmost

image

definition

and

contrast.

2. Illumination

1) Checkingthe illumination

Unevenness in the illumination

will show

conspicuously

in photomicro-

graphy than in observation. Consequently,

before taking a photograph,

recheck the

positioning

and centering of the lamp and

the correct adjustment of the condenser.

2) Selection of voltage and filter

The color temperatu re of the Iight source

varies with the voltage being used. There-

fore,

color

photomicrography,

the

selection of voltage and filter

is essential

(for the result to be obtained).

In color photomicrography,

set the bright-

ness control

dial to 5.5, and use NCB10

filter.

Depending

upon the make of the film,

different

color renditions may result. It is

recommended that in addition to the NCB

10 filter

a color compensation

filter (CC

filter),

available from the film

manufac-

turer, be used.

3. Shutter Speed

Desirable shutter speeds for least vibration are

1/4 ~ 1/15 sec. Adjustment of the image bright-

ness for

color

photomicrography

should be

made by means of the NO filters.

Some specimens require, on account of their

insufficient

brightness, longer exposure times,

and consequently

poor color

reproducibility

owing to the "Reprocity

Law Failure" of film

may result. So, when taking picture of such

specimens, it is recommended to use the N ikon

Polarizing Microscope OPTIPHOT-POL.

4. Manipulation of Field and Aperture

Diaphragm

In photomicrography,

the adjustment

of the

field diaphragm is important for the purpose of

limiting extraneous light which causes flare in

the microscope

image. Stop down

the dia-

phragm so as to get an illuminated area slightly

larger than that of the picture field. By adjust-

ing the apertu re diaphragm, a change of depth

of focus, contrast and resolution

of image is

attainable. Select a size suited to the purpose.

Generally speaking, the aperture diaphragm, is

properly

stopped down to 70 ~ 80% of the

aperture of the objective being used.

5. FOC;USing

Focusing for photomicrography

can be done

with

the observation

tube of the trinocular

eyepiece tube "TP"

or by using the Microflex

finder.

1) For focusing with the Microflex finder

Refer to the Instruction

Manual for the

Nikon Microflex.

2) Focusingwith the observation tube

For focusing with the observation tube, use

the eyepiece incorporating the photo mask.

Before proceeding to focusing, the bino-

cular diopter adjustment should have been

finished.

(1) Insert the eyepiece with photo mask into

the eyepiece sleeve on the side of the user's

dominant

eye, and the viewing eyepiece

into the other side sleeve.

Turning the diopter ring, bring the double

cross lines in the mask eyepiece into sharp

focus, and then, turning

the coarse-fine

focus knob, focus the specimen image onto

the focused surface at the center of the