Etaluma LS460 Instrukcja uruchamiania - Strona 11

Przeglądaj online lub pobierz pdf Instrukcja uruchamiania dla Mikroskop Etaluma LS460. Etaluma LS460 14 stron. Lumascopes with lumaview (for use with ls560, ls460, ls600, ls620, ls720)

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1. Lumaquant software offered by Etaluma (see Lumaquant Startup Guide for compiling images into

video,

2. Your own compiling software such as ImageJ, Fiji, MSMoviemaker, or iMovie, or

3. Use of two freeware software programs together, i.e., AviSynth and MeGUI (instructions described in

Appendix C). If using this option, be sure to read the instructions first because the two programs must

be downloaded before image capture.

Time-Lapse

1. To configure a time-lapse assay, the Protocol feature is utilized.

Click the "P" icon in the Left Tool Bar (fourth from the top) to

open its dialog box containing three tabs. Enter information and

values as you move between the tabs. Be sure to click Save

before leaving a Protocol.

2. In the first tab called Information, click the Load Existing button

to bring up a previously saved Protocol (select desired Protocol

and click OK). Click the Clear Existing button if values have been

entered on any of the tabs and not saved. A Save Protocol File

dialog box will open asking if you want to save the current

Protocol. If Yes, click OK. If not previously saved, enter the new

desired Protocol name in the Filename field or click on an

existing File name to overwrite. If the latter, a File Overwrite

Alert will open; enter Yes or No. You are now ready to create a

the Information tab, if you enter User Notes, they will become part of the new Protocol. You can also

enter User Notes for a previously saved Protocol in this tab.

3. Click on the Acquisition tab and check Time-Lapse. Enter the

Interval and Duration information. Before initiating the time-

lapse run, review Computer Configuration in the Help Section

and change your computer power and Windows update settings

so they cannot automatically turn on and interfere with

scheduled image captures. Of particular importance to turn off

are Sleep Mode and Auto Restart. (After time-lapse is

completed, be sure to change the settings back.)

4. Click on the Image tab to select the channels to be used in the

time-lapse and adjust their settings (LS460 will show only the

Brightfield settings). If settings were previously determined in

Manual Image (Section II.D above), transfer the values into the

current Protocol by clicking the Use Manual Settings button. The

desired values can also be entered manually. Note: This dialog box

cannot be used to optimize the image settings because it is not

interactive with the Live Image Window.

If optimization of any of the Channel settings has not been done or is still needed, click on the Manual

Image icon on the Left Toolbar (top icon) to open its dialog box and fine tune the settings with a

2017.11.20

LS560/460 Startup Guide

new Protocol. On