Etaluma LS460 Instrukcja uruchamiania - Strona 8
Przeglądaj online lub pobierz pdf Instrukcja uruchamiania dla Sprzęt laboratoryjny Etaluma LS460. Etaluma LS460 14 stron. Lumascopes with lumaview (for use with ls560, ls460, ls600, ls620, ls720)
Drukuj stronę1700 x 1700 pixels but corners will be darker. If faster frame rates are
needed, use smaller frame sizes, e.g., 800 x 800 pixels.
If the frame size is changed, there will be a prompt to restart Lumaview when
setup is completed and the OK is clicked at the bottom of Instrument Setup.
It is important to shut down and reopen the software to ensure the
frame size is actually changed. Otherwise the new frame size may
show in Instrument Setup but it may not actually be different.
Always check the lower left status bar of Live Image Window to see
the actual frame size.
5. Filename root: Enter desired file name root for all images in this imaging session. Select the file format
desired (Tiff is recommended).
6. The Time-lapse folder default is given but can be changed if desired. Time-lapse is set up under
Protocol as described in Section II.I below. (Note: the folder name Lumaview720 does not affect use of
the software for the LS560 or LS460).
7. Select the time in seconds for the LED to be on before image snap. One second is typically used for
fluorescence. Check Auto Composite (LS560 only) to have images in multiple channels automatically
composited and saved in a Composites folder. Check Individual Channel Folders to have images from
multiple channels in a Protocol saved in separate folders. If only 1 channel is selected in Protocol and
the Individual Channel Folders is checked, the images will be in a sub-folder entitled with the channel
name.
Of the next 5 features listed on the left, check those desired:
a. Time/date label will be displayed in the lower left corner of each image.
b. Hot pixel removal. Check if desired and then complete Instrument Setup. (To start the process,
click Utilities in the title bar and from the pull-down menu select Detect Hot Pixels to open its dialog
box. Information on next steps is available in the Help section.)
c. Pseudo color FL channels (LS560 only) results in live and snapped fluorescent images colored green
according to signal intensity.
d. "Click" sound for manual image snap results in a "camera shutter-like" sound whenever a manual
image is snapped.
e. Manual image autosave results in images being saved
automatically without the Save As dialog box opening each
time. Each image is numbered according to DOYHHMMSSmm
where DOY is day of year, HH is hour in 24 hour time, MM is
minutes, SS is seconds, and mm is milliseconds. Thus each
image number is unique and in numerical order.
2017.11.20
LS560/460 Startup Guide
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