cytiva pH/C-902 Instrukcja obsługi - Strona 3

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Step

Action

Stop the flow and wait 15 minutes until the temperature is

constant in the range of 20-30℃.

Read the displayed conductivity value and compare it to the

theoretical value at the temperature of the calibration solution

from the graph below.

No further actions are necessary if the displayed value and the

theoretical value correspond. If not, proceed with the following steps:

Select System:Calibrate.

Result: The Calibration dialog box opens.

In the Monitor box, select CIT101_Cal or CIT102_Cal.

Type the theoretical conductivity value according to the graph

below in the Reference Value 1 box.

Click the Read Value 1 button.

Result: The measured value is displayed in the Measured

value box. The new constant value is updated and displayed in

the Cell constant box.

Click the Close button to exit the dialog box.

Fig 1. Conductivity of 1.00 M NaCl at 20-30℃

pH/C-902 Instruction 14003958 AB

Preparing and inserting the pH electrode

The tip of the pH electrode consists of a thin glass membrane.

protect it from breakage, contamination and drying out or the

electrode will be destroyed. Always store the electrode with the end

cover filled with a 1:1 mixture of pH 4 buffer and 1 MKO

in water only.

When it is not in use, e.g. during system CIP, service or storage of the

system, the pH electrode is replaced by a pH plug and the pH

electrode is placed in a holder that is attached to the frame adjacent

to the pH monitor

The instruction below describes how to insert the pH electrode:

Step

Action

Unscrew the plastic fastening nut from the pH cell and remove

the pH plug.

Remove the pH electrode from its holder and take off the

protective cap. rinse the electrode with pure water.

Note:

The pH plug may be placed in the pH electrode holder while the

electrode is in use

Remove the fastening nut from the pH plug, insert the

electrode into the electrode adaptor and slide the nut over the

adaptor.

Carefully insert the electrode in the flow cell. Tighten the nut by

hand to secure the electrode.

Connect the monitor cable to the electrode.

Connect the grounding wire to the pH cell.

Fig 2. Inserting pH electrode into the flow cell

Note: if the electrode is not fully inserted, the system will leak and a

dead volume will occur in the holder.

CAUTION

Handle the pH electrode with care

Note: When running chromatography using organic solvents it is

recommended that the pH electrode is removed and the

dummy electrode is inserted in its place, as organic solvents

will cause pH electrode degeneration.

. Do not store