10x Genomics Visium CytAssist Referência rápida - Página 7

Procurar online ou descarregar pdf Referência rápida para Equipamento de laboratório 10x Genomics Visium CytAssist. 10x Genomics Visium CytAssist 8 páginas.

10x Genomics Visium CytAssist Referência rápida
Visium CytAssist
Load Slides onto Slide Stage
Load Tissue Slides onto Tissue Slide Stage
a. Press down on the wide end of clip to lift
and pivot the narrow end of the clip. Pivot
the clips to their outermost position.
b. Lay the tissue slide flat against the stage
surface. Both hands needed: use one hand to
hold the slide in place and the other to pivot
the clips and overlap the slide.
c. Use fingers to finely adjust the position of the
tissue/sample within the alignment guides.
B
Pivot clip
Secure slide
d. If only one tissue on one slide will be
loaded, use a blank slide for the second
position on the Tissue Slide Stage.
CORRECT
Tissue lies completely within
alignment guides
6.5 mm capture area used as an example
Quick Reference Cards |
CG000543 | Rev A
Press down on the wide
end of the clip
C
INCORRECT
Tissue is intersected by
alignment guides
Visium Slides
The Visium Slide Stage holds one of the
following at a time:
• Visium CytAssist Spatial Gene Expression
Slide v2, 6.5 mm or 11 mm
• Visium CytAssist Training Slide
• Visium CytAssist Calibration Slide
Load Tissue Slides onto Visium Slide Stage
a. Inspect the Visium Slide Stage for dust/debris.
b. Open the Visium Slide Lock by using one
finger to pivot.
c. Line up the slide with the label face up and
oriented to the right.
d. Fit the slide within the raised grooves on
the left, top and bottom.
e. Secure the slide by pivoting the Visium
Slide Lock gently to the left. The lock will
partially obscure the slide label when
correctly secured.
Visium Slide Stage is Empty
INCORRECT
Slide does not fit inside grooves.
Capture areas do not line up
with alignment windows.
Visium Slide Stage is Loaded
Correctly with One Slide
INCORRECT
Label is on the left.
Capture areas do not line up
with alignment windows.
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