Amersham Pharmacia Biotech INV-907 Manual de instruções - Página 3

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Manual sample loading
The sample loop and Superloop can be loaded
with a syringe. Connect Superloop with the
bottom tubing connected to port 2 and the
upper tubing to port 6.
For large volumes use a luer
female/1/16" male union
connected to port 3.
For smaller volumes use an
injection needle together with
the injection fill port as
described below:
Check the fill port nozzle is screwed into
the fill port.
Note: The thread is left-handed.
1 Loosely thread the fill port screw into
valve port 3.
2 Insert the injection needle (0.7 mm o.d.)
into the injection fill port.
3 Tighten the fill port until the nozzle has
formed a seal around the needle tip. When
the seal is adjusted correctly, it feels as if
you are penetrating a septum at the end of
the injection fill port. The seal should
provide easy insertion and removal of
the needle.
4. Mount the syringe holder on the fill port.
Valve INV-907
Instructions 56-8101-01 Edition AC
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IN V -9 0 7
Two techniques can be used for filling
the sample loop; partial or complete filling.
Type of filling
Volume to load
Partial filling
max 50% of the sample loop
volume
Complete filling
2-5 times the sample loop
volume
Partial Filling
Partial filling is used when high recovery is
required. Partial filling allows the injected
volume to be changed without changing
the loop and does not waste sample.
Partial filling is achieved as follows:
1 Set injection valve to position LOAD.
2 Load the syringe with a large volume of
buffer (5x the loop volume).
3 Fill the sample loop carefully with buffer.
4 Set the injection valve to position INJECT
before taking out the syringe.
Note: If the syringe is taken out when
the injection valve is in position LOAD,
self drainage will occur and air will enter
the sample loop.
5 Load the syringe with the required volume
of sample. No more than half (50%) a
loop volume of sample should be loaded
into the loop.
6 Insert the syringe in position 3 on
the injection valve. Set the injection valve
to position LOAD.
Note: Do not load the sample before
the valve is in position LOAD.
7 Gently load the syringe contents into
the loop.
8 Leave the syringe in position. The sample
will be injected onto the column when
the valve is switched to INJECT in
the method.
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