BD FACSCanto II Manual de referência rápida - Página 3
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Optimize settings, if needed.
•
To adjust the optimization gate, click
needed adjustments, and then click
•
To adjust detectors, thresholds, or spectral overlap settings, click on
the corresponding tab and use controls to adjust the settings.
3
If there are more tubes for that panel type, click
Otherwise, click
Report for optimized settings.
4
Click
Optimizing Instrument Settings: BD FACSDiva Software
Before you record data for a sample, optimize the PMT voltages, compensation,
and threshold settings for the sample type and fluorochrome.
1
Verify the instrument configuration.
2
Create an experiment.
3
Select the experiment-level settings, and change, add, or delete
parameters as needed.
4
Apply setup results.
•
Right-click the experiment-level settings, and choose Apply Setup.
•
Select a setup, and click Apply.
5
Create compensation controls; click OK to remove the setup link.
•
If needed, edit labels for the Compensation Controls.
6
Optimize instrument settings using an unstained control sample.
•
Place the current tube pointer next to the unstained control tube,
install the tube, and click
•
Adjust FSC and SSC voltages, and the FSC threshold, if needed.
•
On the Unstained Control worksheet, adjust the P1 gate, and apply
gate changes to the remaining compensation controls.
•
Set the fluorescence histograms to show grids for each log decade.
•
Optimize the voltages to place the negative population for each
fluorescent parameter within its first log decade.
.
, and then view and print the Setup
, remove the tube (if prompted), and then click
.
3
, make the
.
, and then
.