4Deep Desktop Microscope Manual do utilizador - Página 5

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4.2 Sample Stage

The sample stage has two separate control

knobs, one located next to the laser power

switch which controls the vertical (z) transla-

tion stage shown in Fig. 4, and a second set

of vertically aligned knobs on the other side of

the microscope which control the x-y transla-

tions of the stage, in Fig. 4(a).

The sample stage has several custom sample holders available, which are designed to hold

standard microscope slides or sample dishes. The magniﬁcation of the microscope is depen-

dent on the Source to Object Distance (SOD), and the vertical position of the sample stage

controls this. Warning: Be careful of the point source when moving the vertical sample stage

position; DO NOT touch the point source.

(a) The x-y controls

Figure 4: The z control knob for the sample stage

Increase SOD

Magniﬁcation Decreases

Decrease SOD

Magniﬁcation Increases

Located in the lower portion of the microscope (Fig. 5), the point source is made of an IIIB

405 nm laser, a 60x objective and a pinhole a similar size to the wavelength of the laser. The

pinhole is on the exposed platinum foil shown, and is extremely fragile. Please refer to the

"General Safety Information" at the start of this manual.

Figure 5: Point source assembly