Active Motif PIXUL Manual do utilizador - Página 11

Procurar online ou descarregar pdf Manual do utilizador para Equipamento médico Active Motif PIXUL. Active Motif PIXUL 15 páginas. Multi-sample sonicator

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chosen to have the longest process time. For example, a plate with the following column-
associated process times will take a total of 21 minutes:
Columns
1, 2 and 4
3, 6, 9, 10 and 12
5, 7 and 8
11
Burst Rate: The frequency with which the burst (a set of pulses) transitions from one column
to the next; this parameter is always the same for all columns.
We recommend using the following parameter specifications as a starting point, and adjust-
ing Process Time to optimize for your specific sample and application:
Sonication Parameter
Pulse [N]
PRF [kHz]
Process Time
Burst Rate [Hz]
*Labile marks, like phosphorylated epitopes, may be preserved better by discontinuous
sonication (e.g. rather than 30 minutes, 4 rounds of 4-6 minutes each, where the PIXUL
Coupling Fluid is allowed to circulate and cool between runs).
**Please see our Quick Guides for additional details on sonication recommendations.
Warning: To avoid damage to the instrument transducers DO NOT exceed: a maximum Pulse
of 50 or a maximum PRF of 1.00, and DO USE a minimum Burst Rate of 20.00.
8. Once the PIXUL Coupling Fluid has reached approximately 15°C, press Start on the touchscreen.
The time to completion will appear in the top left hand corner of the touchscreen.
9. Once the run has completed, open the external lid. PIXUL Coupling Fluid will drain from under-
neath the sample plate for the next few seconds.
10. Unload the sample plate.
11. To turn off the PIXUL instrument, press the main power button on the front side of the PIXUL
instrument and switch the power switch off on the back side of the PIXUL instrument.

Maintenance

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Process Time
18 minutes
12 minutes
21 minutes
0 minutes
DNA**
(Cells & Tissue)
50
1.00
30:00
20.00
8
Chromatin**
(Cells & Tissue)
50
1.00
30:00*
20.00
DNA/RNA**
(Purified)
50
1.00
10:00-30:00
20.00