Automate ValveLink 8 Manual do utilizador - Página 8

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Chromatography

Gel/Blot Washing System

vacuum trap

rocker

Without a pump, fl ow rate

is determined by relative

height of reservoirs.

1) Position reservoirs above

column ringstand.

2) Attach valve unit to col-

umn stand.

3) Run tubing from upper

valve barbs into res er -

voirs.

4) Connect short pieces of

tub ing between lower valve

barbs and man i fold.

5) Connect manifold outfl ow

to your pump with 1/16"

Fraction

i.d. tubing.

collector

6) Pump outfl ow connects to

or waste

column inlet as usual.

7) Plug numbered valve ca bles

Flow rate is determined by

relative height of reservoirs.

1) Position reservoirs above

washing area.

2) Attach valve unit to ring-

stand.

3) Run tubing from upper valve

barbs to reservoir nipples.

4) Connect tube from lower

valve barbs to tray inlets.

5) Connect tray outfl ow to

fourth upper valve barb.

6) Connect this valve's out fl ow

to vacuum trap.

7) Plug numbered valve ca bles

With a ValveLink8, a researcher can turn valves on and off

using eight TTL pins

son al com put er and their own custom programs. (See the de-

scrip tion of Mode #1 in the next chapter to accomplish this using

only three or four TTL outputs.) The ValveLink will open and close

valves mirroring the com put er signals (low = off, high = on) with

sub-10 mil li sec ond ac cu ra cy. ValveLink16's have one additional

TTL input to 'multiply' the eight com mon inputs by 2 into 16 full

channels of valve control.

ValveLink8

[Back panel diagram view]

Standard female DB-9 connector

Pins 5,4,3,2,1 = TTL inputs 0-4

Pin 6 =

ground

Pins 9,8,7 = TTL inputs 5-7

Manual Control

Each press of a front-panel pushbutton toggles its valve on

and off. The 'Reset' button immediately turns ALL valves off and

resets the ValveLink's internal circuitry. The ValveLink16 includes

16 valve status LED's, but has only eight pushbuttons like the

ValveLink8. An up/down switch tog gles the ValveLink16's push-

buttons between control of two 'banks' of eight valves.

3-way Valves

The ValveLink also supports 3-way, single-solenoid valves (2

wire). Program the ValveLink as you normally would for 2-way

valves, after determining which of two inlets or outlets are opened

with the on and off states.

Operation

from an interfacing board on a per-

ValveLink16

Female DB-15 connector

Pins 8,7,6,5,4 = TTL inputs 0-4

Pin 12 = ground

Pins 15-13 =

Pins 3 = TTL bank

TTL inputs 5-7

9 9

select