CORNING 431485 Dados técnicos e instruções de funcionamento - Página 3
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2. Prerinsing
Membranes fitted to Spin-X
UF concentrators contain trace amounts of glycerin and
®
sodium azide. Should these interfere with analysis they can be removed by rinsing fill
volume of buffer solution or deionized water through the concentrator. Decant filtrate
and concentrate before processing sample solution. If you do not want to use the pre-
rinsed device immediately, store it in the refrigerator with buffer or water covering
the membrane surface. Please do not allow the membrane to dry out.
3 . Sterilization of Polyethersulfone Membranes
Polyethersulfone membranes should not be autoclaved as high temperatures will
substantially increase membrane MWCO. To sterilize, use a 70% ethanol solution
or sterilizing gas mixture.
Technical Specifications
Table 1. Technical Properties
Spin-X UF 6
Concentrator Capacity
Swing bucket rotor
Fixed angle rotor
Dimensions
Total length
Width
Active membrane area
Hold up volume of membrane
Dead stop volume*
Materials of Construction
Body
Polycarbonate
Filtrate vessel
Polycarbonate
Concentrator cap
Polypropylene
Membrane
Polyethersulfone
*Dead stop volume as designed in molding tool. This volume may vary depending on sample, sample
concentration, operation temperature and centrifuge rotor.
4
Spin-X UF 20
6 mL
20 mL
6 mL
14 mL
122 mm
116 mm
17 mm
30 mm
2
2
2.5 cm
6.0 cm
<10 µL
<20 µL
30 µL
50 µL
Polycarbonate
Polycarbonate
Polypropylene
Polyethersulfone
Table 2. Maximum Recommended Spin Force (xg)
Swing Bucket
Spin-X
®
UF 6
5,000 to 50,000 MWCO PES
100,000 MWCO PES
Spin-X UF 20
5,000 to 50,000 MWCO PES
100,000 MWCO PES
Table 3a. Performance Characteristics Spin-X UF 6
(Time to concentrate up to 30x [min.] at 20°C and solute recovery %)
Rotor
Start Volume
Min.
BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
20
10,000 MWCO PES
13
30,000 MWCO PES
12
IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES
18
50,000 MWCO PES
17
100,000 MWCO PES
15
Table 3b. Performance Characteristics Spin-X UF 20
(Time to concentrate up to 30x [min.] at 20°C and solute recovery %)
Rotor
Start Volume
Min.
BSA 1.0 mg/mL (66,000 MW)
5,000 MWCO PES
23
10,000 MWCO PES
16
30,000 MWCO PES
13
IgG 0.25 mg/mL (160,000 MW)
30,000 MWCO PES
27
50,000 MWCO PES
27
100,000 MWCO PES
25
Fixed Angle
4,000 xg
10,000 xg
4,000 xg
6,000 xg
5,000 xg
8,000 xg
3,000 xg
6,000 xg
Swing Bucket
25° Fixed Angle
6 mL
6 mL
Rec.
Min.
Rec.
98%
12
98%
98%
10
98%
98%
9
97%
96%
15
95%
96%
14
95%
91%
12
91%
Swing Bucket
25° Fixed Angle
20 mL
14 mL
Rec.
Min.
Rec.
99%
29
99%
98%
17
98%
98%
15
98%
97%
20
95%
96%
22
95%
91%
20
90%
5