Olympus BX3-RFAS Manual de instruções - Página 13
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Dichroic Mirror and Filter Configurations of Fluorescence Mirror Units
Excitation
Mirror unit
method
U-FUW
U
U-FUN
V
U-FVN
BV
U-FBVW
U-FBW
B
U-FBN
G
U-FGW
Y
U-FYW
Combinations for color separation
U
U-FUNA
U-FBWA
B
U-FBNA
U-FGWA
G
U-FGNA
Exclusive combinations for fluorescent proteins
CFP
U-FCFP
GFP
U-FGFP
YFP
U-FYFP
RFP
U-FRFP
mCherry
U-FMCHE
Dichroic mirror
Excitation filter
BP340-390
DM410
BP360-370
DM455
BP400-410
DM455
BP400-440
BP460-495
DM505
BP470-495
DM570
BP530-550
DM600
BP545-580
DM410
BP360-370
BP460-495
DM505
BP470-495
BP530-550
DM570
BP540-550
DM455
BP425-445
DM490GFP
BP460-480
DM515
BP470-500
DM565HQ
BP535-555HQ
DM595
BP565-585
Barrier filter
· Autofluorescence observation
BA420IF
· DAPI: DNA staining
· Hoechest 33258, 33342: Chromosome
· Catecholamine observation
BA460IF
· Serotonin observation
· Tetracyline: Bones, teeth
· Quinacrine, quinacrine mustard:
Chromosome
BA460IF
· Thioflavine S: Lymphocyte
· Acriflavine: Nucleic acid
· ECFP
· FITC: Fluorescent antibody
· Acridine orange: DNA, RNA
BA510IF
· Auramine: Tubercle bacillus
· EGFP, S65T, RSGFP
· Rhodamine, TRITC:Florescent antibody
BA575IF
· Propidium iodide: DNA
· RFP
BA610IF
Texas Red: Fluorescent antibody
For observing only the U-excitation stain
BA420-460
when using U-excitation stain together
with FITC.
For observing only the B-excitation stain
BA510-550
when using B-excitation stain with TRITC
or Texas Red.
For observing only the G-excitation stain
BA575-625
when using G-excitation stain together
with Cy5.
BA460-510
BA495-540
BA515-560
BA570-625HQ
BA600-690
Applications
For ECFP.
For EGFP.
For EYFP.
For RFP.
For mCherry.
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