Olympus BX45 Manual de instruções - Página 20

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Aperture iris

diaphragm image

Objective pupil

Compatibility of Objectives and Condensers

* When using a 4X or lower-power objective, fully open the condenser aperture iris diaphragm and use the field iris

diaphragm in the base as aperture diaphragm. With the 1.25X to 2X objectives, the peripheral sections of the field of view

may be dark but observation is still possible.

}If you want objectives for cytological observation, please purchase the PlanN10XCY and PlanN series objectives or the

PlanFLN10XCY and UPlanFLN series objectives.

70-80%

30-20%

Fig. 26

N.A. scale

Fig. 27

Objective Magnification

1.25X - 4X *

4X -60X

100X

Aperture Iris Diaphragm (Figs. 26 & 27)

· The aperture iris diaphragm determines the numerical aperture of the

illumination system. Matching the numerical aperture of the illumination

system with that of the objective provides better image resolution and

contrast, and also increases the depth of focus.

· Since the contrast of microscope specimens is ordinarily low, setting the

condenser aperture iris diaphragm to between 70% and 80% of the N.A.

of the objective in use is usually recommended. If necessary, adjust the

ratio by removing the eyepiece and looking into the eyepiece sleeve

while adjusting the aperture iris diaphragm ring @ until the image shown

in Fig. 26 is seen.

}Using the numerical aperture scale:

Set the condenser numerical aperture scale to about 80% of the NA

value ² of the respective objective. (Fig. 27)

Example: With the UPlanFLN40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6.

BX45 Condenser

Applicable by swing the top lens out.

Applicable by engaging the top lens in the light path.