Olympus CX31-P Manual de instruções - Página 17

Procurar online ou descarregar pdf Manual de instruções para Microscópio Olympus CX31-P. Olympus CX31-P 36 páginas. Polarizing microscope

4-6 Condenser

Fig. 17

Field iris diaphragm image

Field of view of eyepiece

Fig. 18

Fig. 19

}When using the U-CTAD centering adapter for objective lens, perform the

operation in

section 5-1 (page 17) before proceeding to the following steps.

1. Engage the 10X objective in the light path, bring the specimen into focus

and rotate the field iris diaphragm ring 1 counterclockwise to stop down

the field iris diaphragm slightly.

2. Turn the condenser height adjustment knob 2 so that the image of the

field iris diaphragm is focused on the specimen surface.

3. Turn the two condenser centering knobs 3 alternately to make the im-

age of the diaphragm concentric with the field.

4. Open the field iris diaphragm so that its image inscribes the field of view.

If the image is eccentric, adjust the centering knobs again.

5. Enlarge the iris diaphragm image until it just circumscribes the field of

view.

}The aperture iris diaphragm adjusts the numerical aperture of the illumi-

nation system. Aligning the numerical aperture 1 of the illumination sys-

tem with that of the objective in use improves the contrast and resolution

as well as the focal depth of the observed image.

How to adjust the aperture iris diaphragm

Set the aperture iris diaphragm lever 2 of the condenser so that its

numerical aperture 1 matches the NA data indicated on the objective.

As the aperture iris diaphragm lever has a certain width, set it so that the

numerical aperture matches at the center of this width.

When the 100X oil-immersion objective is used, set the aperture iris

diaphragm lever 2 to a numerical aperture of 0.9.

}As microscopic specimens in general have low contrast, it may be opti-

mum to set the aperture iris diaphragm to between 70% and 80% of the

aperture number of the objective.

# Stopping down the aperture iris diaphragm excessively may result

in production of ghost.

Adjusting the Centering Adapter for Objective Lens in

CX31-P

(Figs. 17 & 18)

(Fig. 19)