Olympus Fluoview FV1000 Visão geral - Página 16
Procurar online ou descarregar pdf Visão geral para Microscópio Olympus Fluoview FV1000. Olympus Fluoview FV1000 28 páginas. Confocal microscope
Também para Olympus Fluoview FV1000: Manual do utilizador (42 páginas), Instruções breves (7 páginas), Instruções breves (7 páginas), Manual do utilizador (9 páginas), Manuallines (3 páginas)
Imprimir página
Diffusion Measurement Package
This optional software module enables data acquisition and analysis to investigate the molecular interaction
1.5
and concentrations by calculating the diffusion coefficients of molecules within the cell.
1
Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular
130
0.5
sizes and speeds.
Pixels
125
0
105
125
105
130
Pixels
RICS—Raster Imaging Correlation Spectroscopy
Raster image correlation spectroscopy (RICS) is a new method for analyzing the diffusion
and binding dynamics of molecules in an entire, single image. RICS uses a spatial
correlation algorithm to calculate diffusion coefficients and the number of molecules in
specified regions.
Cross correlation RICS (ccRICS) characterizes molecular interactions using fluorescent-
labeled molecules in two colors.
point FCS—Point scan Fluorescence Correlation Spectroscopy
point scan fluorescence correlation spectroscopy (point FCS) analyzes intensity
fluctuations caused by diffusion or binding/unbinding interactions of a protein complex.
point FCS uses an auto correlation function to carry out operations on fluorescence
signals obtained by continuous scanning of a single pixel on the screen.
point scan fluorescence cross-correlation spectroscopy (point FCCS) analyzes the
fluctuation of fluorescent-labeled molecules in two colors. The coincidence of fluctuations
occurring in two detection channels shows that the two proteins are part of the same
complex.
point FCS and point FCCS can now be performed with a standard detector, eliminating
the need for a special high-sensitivity detector.
FRAP Analysis
The Axelrod analytical algorithm is installed as a FRAP analysis method. The algorithm is used to calculate diffusion coefficients and the
proportions of diffusing molecules.
Analytical methods
according to molecule
diffusion speeds
point FCS
Capable range
RICS
of measurement
FRAP
Small molecules
Proteins
in solution
in solution
> 100
~ 100
Diffusion of
Lateral diffusion
proteins
in cell membrane
in cell
1 ~ 100
< 0.1
15
Protein
Molecular complex
trafficking
formation,
aggregation
< 0.01
<< 0.001