Olympus Fluoview FV1000 Manual do utilizador - Página 38
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Troubleshooting Guide
Irregularity
2. Fluorescence image
cannot be observed.
3. Transmitted image cannot
be observed.
4. Image is disturbed.
5. Reflected light (laser light)
enters the fluorescence
image.
6. Fluorescence image is
poor.
7. Fluorescence image is
dark and noisy.
III . TROUBLE Q&A
III. 1 - 2
Page
Cause
The offset value is too large.
The detector for the channel to be
detected is not selected.
The transmitted light detection
channel is not selected.
The transmitted light filter for the
microscope is in the light path.
The PMT voltage of the transmitted
light detection channel is too low.
The offset value for the transmitted
light detection channel is too large.
The system installation location is
subject to excessive vibrations.
Extraneous light such as the light of a
fluorescent lamp is detected.
The barrier filter is set erroneously or
absent.
The set acquisition wavelength is
overlapped with or too close to the
excitation laser wavelength. (Spectral
detection system only)
The barrier filter that can cut the
wavelength of the laser light
irradiated from the ASU (auxiliary
scan unit) is not selected. In the case
of a spectral detection system, the
acquisition wavelength setting may
be inappropriate.
The front lens of the objective is dirty. Clean the objective front lens by wiping it
When an objective with correction
collar is in use, the correction collar is
adjusted improperly.
The cover glass thickness is
inappropriate.
The laser beam is too weak.
The fluorescent dyeing method and
excitation wavelength do not match
each other.
Remedy
Decrease the offset value.
Select the detector.
Select the transmitted light detection
channel.
Disengage the filter from the light path.
Increase the PMT voltage.
Decrease the offset value.
Contact Olympus.
Turn the room light low before acquiring
image.
Engage a barrier filter that can cut the
excitation laser wavelength in the light
path.
acquisition wavelength that is
not interfered with by the laser
wavelength. (Note that, when the confocal
pinhole is large and the BS20/80
excitation DM is used, penetration of laser
light may become large.)
Engage a barrier filter that can cut the
laser wavelength from the ASU in the light
path. With a spectral detection system,
change the acquisition wavelength
setting.
with a piece of gauze.
Adjust the correction collar properly.
Use a cover glass with thickness of 0.17
mm.
Increase the laser intensity.
Select a laser optimum for the fluorescent
dyeing method.