Olympus FV3000 Manual de operação - Página 13
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XYT Image Acquisition
(with the Z drift compensation）
⑩
【Attention】
If [Rest] is shorter than 31 seconds, Z drift
correction works only first scanning. For
every time working, set the [Interval] so
that the [Rest] to be 31sec or longer.
⑤
⑥
⑦
⑧
③
④
＊In advance, prepare to use ZDC.
（See page 22 for details. ）
＊Set for acquiring the series image.
Setting Near limit
③ Select [Microscope] in [Tool Window] menu.
Press the
set the Near limit to all objective lenses in
[Z limit Setting].
④ Pressing the
position as 0 position.
ZDC Setting
⑤ Set [ZDC DM] in [ZDC Control] on
[Microscope] Tool Window to "In".
⑥ Specify the search zone of the coverslip
position in [Upper Limit] / [Lower Limit] in
[Search Zone] .
⑦ Set [Z Drift Compensation in series scan] in
[ZDC Control] in [Microscope] Tool Window
to "ON".
⑧ Adjust focus and press the
button of [Coverslip position]. The coverslip
top surface position is acquired.
When the Z drift compensation is
successful, the buzzer sounds beeps once
only. If the Z drift compensation is not
successful, the buzzer sound beeps three
times.
⑧ When performing the Z drift compensation
continuously during series scan, press the
button in [ZDC Control] in
[Microscope] Tool Window. As this function
performs the drift compensation in real-
time during series scan, the focusing can
be at a high-speed.
・High-speed scanning →Use continuous AF
・If Rest time is longer then 30 seconds
→You had better not use Continuous AF
：Don't operate ⑦.
⑨ Press
in [Normal] tab in [Acquire]
Tool Window to start acquisition
◆ZDC◆
button to
button to current
.
9