Olympus IX71 Brochura e especificações - Página 7

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Nomarski DIC system offers the choice of optimal resolution or

high contrast in live cell observation.

DIC

Differential Interference Contrast

Live cells specimens vary in thickness from from that of a nematode

worm such as C. elegans to a monolayer of cultured cells. The

requirements for DIC are also varied according to the specimen

from thinner cells being almost invisible to thicker specimens having

a lot of inherent contrast. Olympus provides three DIC systems with

varying amounts of shear. Small shear, high resolution sets are

excellent for thicker specimens. High contrast prism with twice the

normal shear are excellent for very thin specimens.

Selecting the optimum DIC prism optimum for specimen

thickness and objective magnification

Thin specimen

(Big shearing value)

U-DICTHC

for superior contrast with thin specimen

observation

U-DICT, U-DICTS

for general observation

U-DICTHR

for superior resolution with thick specimen

observation

Thick specimen

(Small shearing value)

10X

40X

100X

DIC sliders

High resolution DIC slider for transmitted

High contrast DIC slider for transmitted

light/U-DICTHR

light/U-DICTHC

Comparison of thick specimen (C. elegans), showing differences in shearing value

DIC observation using U-DICTHR

Comparison of thin specimen, showing differences in shearing value

DIC observation using U-DICTHC

Long working distance universal condenser/IX2-LWUCD

Combining a long working distance (27 mm) and a high numerical

aperture (N.A. 0.55), the LWUCD condenser accommodates most

incubation chambers and T-Flasks. The 5-position turret provides

versatility with DIC or phase inserts. DIC components are specially

designed to obtain high-contrast, high-resolution images with 20X

and 40X objectives.

• New DIC system gives a wider choice

UIS2 expands the selection of DIC

applicable objectives. Each condenser

prism is compatible with more lenses

making setup and configuration easier.

Shift DIC sliders for transmitted light/

U-DICTS

DIC sliders for transmitted light/U-DICT

HR/HC optical elements for IX2-LWUCD

and applicable objectives

DIC elements

IX2-DIC20HR UPLSAPO20X

IX2-DIC20HC UPLFLN20X

IX2-DIC40HR UPLSAPO40X2

IX2-DIC40HC UPLFLN40X

General type optical elements for

IX2-LWUCD and applicable objectives

DIC observation using U-DICT/ U-DICTS

DIC elements

IX2-DIC10

IX2-DIC20

IX2-DIC40

IX2-DIC60

IX2-DIC100

DIC observation using U-DICT/ U-DICTS

Water immersion DIC condenser/IX2-DICD

High performance DIC condenser designed

for excellent optical performance and

specimen access in high magnification

observations. Designed for specimen

access, all controls are front mounted

including prism exchange and aperture

control. Three high numerical aperture top

lenses are available including the water

immersion IX2-TLW that offers 0.9 N.A.

with 3.7 mm of working distance and a 40°

approach angle for micro manipulations.

Top lens combination

Numerical Aperture Working Distance

(N.A.)

(W.D.)

IX2-TLW

0.9

3.7 mm

U-TLD

0.9

1.5 mm

U-TLO

1.4

0.63 mm

Condenser adapter/IX-ADUCD

This is the condenser adapter for upright

microscope condensers on the IX2,

including the 8-position turret condenser

(U-UCD8-2) for maximum system flexibility.

This combination allows the use of various

optional element with high N.A., just

rotating the smooth turret for switching

them easily. The IX2 illumination pillar also

offers a 'condenser-only' tilt mechanism to

quickly allow access to the

specimen without tilting

the entire illumination pillar.

* IX2-TLW cannot be used for U-UCD8-2

Applicable objectives

LUCPLFLN20X

UPLFLN40XO

LUCPLFLN40X

Gliding stage/IX2-GS

Applicable objectives

The Gliding Stage was designed for quick

UPLSAPO10X2

rotation of the specimen using your

UPLFLN10X2

UPLSAPO20X

fingertips. With 20 mm of X-Y travel,

UPLFLN20X

360 degree rotation and completely flat

LUCPLFLN20X

UPLSAPO40X2

surface, a specimen such as the nematode

UPLFLN40X

worm C. elegans can be quickly brought into

UPLFLN40XO

LUCPLFLN40X

the correct position and alignment for

PLAPON60XO

injection or micromanipulations.

UPLFLN60X

UPLFLN60XOI

LUCPLFLN60X

UPLSAPO100XO

UPLFLN100XO

UPLFLN100XOI

Immersion

40°

Water

Oil

Water immersion DIC condenser IX2-DICD + water immersion

top lens IX2-TLW

IX2-GS

New DIC system

Simple principle of Nomarski DIC

microscopy

Nomarski DIC amplifies contrast by using the

phase difference which occurs when light

passes through material with different

refraction or thickness value (e.g. a cell) in a

particular medium (e.g. water). The wave

direction of light from the microscope light

source is unified in a polarizer (condenser

side); and when it passes through the

condenser side DIC prism, it separates into

two beams which cross each other at right

angles. The distance of separation is called

the shearing amount. When two such

separated beams pass through a medium

with different refraction values (e.g. a cell), one

of them is delayed; and when the two beams

are re-composed by DIC prism (the observa-

tion side) and pass through the analyzer, the

interference effect produces the contrast.

This is the principle of Nomarski DIC.

Polarizer

DIC prism

Condenser

Specimen

Shearing value

Objective

DIC prism

Analyzer

Olympus has developed the most suitable

DIC prisms for different types of specimen,

based on the shearing amount. When DIC

contrast is low, the specimen is hard to

observe, while high contrast also hinders

observation because of excessive glare.

Olympus has therefore developed three

different types of DIC prisms to ensure clear

observation for every kind of specimen.