Olympus IX81 Brochura e especificações - Página 11

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Maintaining long-term stability for live cell observation.
Live cell imaging system
Focus drift compensation function for
time-lapse experiments.
Motorized inverted research microscope with
focus drift compensation/IX81-ZDC
This landmark microscope model makes it easy to reproduce any
preset focus position. 785 nm weak laser light is introduced
through the additional optical path between the tube lens and an
objective to measure the distance between the objective and the
reflection plane, which is normally the boundary of the reflective
index difference such as the boundary between cover glass and
cell. Therefore this system never cause unnecessary
photobleaching of the specimen.
19
A.
B.
C.
Closer
Focusing
Further
than focus
position
than focus
position
position
Reflection
plane
Objective
Light
shielding
plate
Laser light
source
(class 1)
Telan lens
Split detector
* Extended image
Accessories to improve stability in long-duration observations
[ CO
incubators/MIU-IBC-I, MIU-IBC-IF ]
2
Highly precise incubator control keeps the environment inside a
laboratory dish completely stable, at just below 37°C temperature,
90% moisture and 5% CO
concentration (when using a CO
2
concentration bomb); in this way, live cell activity can be maintained
for about 2 days. A special designed structure is employed to
minimize the focus drift during temperature control. This is the ideal
solution for time-lapse experiments under both a confocal laser
scanning microscope and a wide field
observation. The opening hole located on
the top heater is available for the cell
injection.
* Built-in stage warming plate
* Objective heater
* 5% CO2 supply tube with ø4 outer diameter, ø2 inner diameter
and 400 mm length.
* Not available in some areas
MIU-IBC-I
MIU-IBC-IF
Basic configuration for control of heaters for
High grade configuration with a built-in
top, bath, stage and objective.
flowmeter for 5% CO
and 95% air. Use the 5%
2
CO
and 95% air bombs.
2
[ Thermoplate/MATS series ]
This thermoplate maintains the
temperature of the sample at 37°C.
* Tokai Hit Company products
[ Nosepiece stage/IX2-NPS]
This simple mechanical stage is designed for long time observations to minimize the
distance change between the specimen and the objective in other words 'focus drift'.
It works by minimizing the effect of temperature change and prevents blur during long
observations. Attach one objective in use.
[ Comparison of normal observation images ]
Change of focus when environmental temperature (25°C )
changes by ±5°C.
*When the microscope is used without an incubator.
Environmental temperature
20°C
25°C
Normal observation images
With IX2-NPS
Live Cell Imaging System
[ Incubators ]
This box type incubator keeps the microscope temperature stable
with enclosing many components inside the box. Please see your
local supplier for more detail.
5%
2
[ Frame plate adapter/
IX2-FP ]
This is used to fix the
microscope frame
to the anti-
vibration stand.
*Screws (available
separately) are required for
fixing.
[ Comparison of fluorescence images ]
Time-lapse observation images.
*When the microscope is used without an incubator.
30°C
Elapsed time
0
30
Normal observation images
With IX2-NPS
60
120 min
20