Olympus R1 Manual de instruções - Página 16

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Olympus R1 Manual de instruções

2.3.1 Settings: Staining Options

Users can select for the use or absence of trypan blue for cell counting with the R1.
Option
With Trypan Blue
*
Without Trypan Blue
*
The R1 is optimized for use with trypan blue. Low contrast from not using trypan blue may lead to suboptimal results.
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The dilution factor will not change automatically. Upon changing the Staining Options, users must manually adjust the dilution factor
accordingly. Failure to adjust the dilution factor will lead to an inaccurate calculation of cell concentrations.
Press [Staining Options] in the Settings screen.
The selected option will be marked with a blue
Change the staining option by pressing the unselected option.
Description
This option is used when cell samples are mixed 1:1 with 0.4% trypan blue for
regular bright field counting. This option generates cell viability data.
Ensure that the dilution factor in your set protocol is set to "2".
When samples are not mixed with trypan blue, turn on this option and follow the
directions in the message boxes.
Ensure that the dilution factor in your set protocol is set to "1".
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