Olympus R1 Manual de instruções - Página 22
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Do not turn the instrument off during this process.
Mix one part 0.4% trypan blue stain with an equal volume of distilled water, PBS, or plain medium. Put 10 µL of
the diluted trypan blue stain into the chamber of a new Counting Slide.
A window will appear with directions for Calibration Step 2 when Calibration Step 1 is complete.
Insert the Counting Slide face up and sample-side first into the counting slide port.
Do not insert the Counting Slide facedown.
Press Start.
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