Olympus U-UCD8A Manual de instruções - Página 18

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9. Rotate the prism control knob of the DIC slider to adjust the contrast of the background color as discussed

below.

a) Rotate the prism control knob of the DIC slider to obtain the background interference color that can achieve the

maximum contrast according to the specimen under observation.

U-DICT:

U-DICTS:

U-DICTHC:

U-DICTHR:

· If the background color is black, darkfield-like observation can be performed.

· If the background color is gray, a 3D-like image with maximum contrast with sensitive gray which is most

sensitive can be obtained.

· If the background color is sensitive magenta, even a minor optical retardation is observed as a color change.

# Care should be taken to keep the specimen surface clean, as even a small amount of contamination on

the surface may show up due to the exceptionally high sensitivity of the DIC method.

b) As DIC exhibits directional sensitivity, the use of rotatable stage is recommended.

}To perform simultaneous reflected light fluorescence and transmitted light DIC observations, refer to the instruc-

tion manual of the reflected light fluorescence system.

# When the PlanApoN60XO, UPlanSApo60XO, PlanApo60XO3 and U-DICTHC are combined (U-DIC60HC

or U-DPO60HC), color irregularities may be noticeable with certain specimens.

The background interference color is continuously variable between sensitive

gray and sensitive magenta.

The background interference color is continuously variable between black and

bright gray.