Nikon A1+ Manual do utilizador - Página 20

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7. You can alter the number of fields of view to be scanned in the Area section and the

number of fields of view on the macro image is automatically updated.

8. The Fields Placement option determines whether the tiling

is around the current stage position or if the current

position is the top left corner of entire tile scan.

9. Set a degree of overlap for stitching in the Overlap section

(10-15%)

10. Select whether the final output is the tiled image or the individual tiles

11. You can select whether the tile scan is automatically saved if you set up a file path

12. To acquire a tiled Z stack enable the Z Series

option

13. Select the number of Z planes and the step size

14. The Z stack will be acquired symmetrically around

the current Z plane

15. If the ultimate aim is to generate a maximum intensity projection of the tiled z stack

then you can just enable the Max IP option. Note that the MIP will be generated

automatically and you will lose the individual z stack images.

16. To begin the tiling experiment press the Scan button