Nikon Eclipse Ci-E Manual rápido - Página 2

Procurar online ou descarregar pdf Manual rápido para Microscópio Nikon Eclipse Ci-E. Nikon Eclipse Ci-E 2 páginas. Bright-field microscopy

9
Focus on the specimen.
Focus on the specimen.
(Continuation)
(Continuation)
( See Chapter 2, Section 2 in the instruction manual for details)
See Chapter 2, Section 2 in the instruction manual for details)
(3)
When the focus was roughly adjusted using coarse focus knob,
turn the fine focus knob to accurately adjust the focus.
(4)
Adjust the brightness of the field of view by turning
the dia-illumination lamp brightness control knob.
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POWER
10
Adjust the diopter.
Adjust the diopter.
( See
See Chapter 2, Section 4 in the instruction manual for details)
Chapter 2, Section 4 in the instruction manual for details)
(1)
Turn the diopter adjustment ring on the right and
left eyepieces to align the end face of the diopter
adjustment ring with the line.
(This is the diopter adjustment reference position.)
(2)
Focus on the specimen using the 40x objective.
(3)
Bring the 10x (or 4x) objective into the optical path.
(4)
Look into the right eyepiece with your right eye and the left
eyepiece with your left eye. Turn the diopter adjustment ring
of each eyepiece to focus on the specimen.
At this point no focus knobs are used.
(5)
Repeat steps (2) through (4) to make sure the focus has been
adjusted properly.
11
Adjust the interpupillary distance.
Adjust the interpupillary distance.
Look into both eyepieces and rotate the binocular part to adjust
the binocular part's opening until the fields of view for the right
and left eyes coincide.
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Notes on controlling the focus knobs
Notes on controlling the focus knobs
Avoid the following actions, which can
cause equipment malfunction.
Rotating the right and left focus
knobs in opposite directions.
Turning the coarse focus knob past
its limit point.
For easy adjustment, look into
the eyepiece as if you were looking
at a distant object.
12
Focus and center the condenser.
Focus and center the condenser.
( See Chapter 2, Section 5 in the instruction manual for details)
See Chapter 2, Section 5 in the instruction manual for details)
(1)
Look into the eyepiece with the field diaphragm stopped down
to the minimum. Focus on the field diaphragm image using
the condenser focus knob, then adjust the condenser centering
screws to center the diaphragm image within the field of view.
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(2)
Bring the 40x objective into the optical path to check the focus
and centering of the field diaphragm image. Make adjustments
in the same way as step (1) as necessary.
(3)
Turn the field diaphragm dial and adjust the field diaphragm image
so that its size is almost the same as the field of view.
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Select the desired objective.
Select the desired objective.
Press the nosepiece forward/reverse rotation buttons, or
the nosepiece address button on the remote control pad to move
the desired objective into the optical path.
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Adjust the aperture diaphragm.
Adjust the aperture diaphragm.
( See
See Chapter
Chapter 2, Section 6 in the instruction manual for details)
Turn the aperture diaphragm lever on the condenser to adjust
Line
the aperture diaphragm so that it is set to 70 to 80% of
the numerical aperture of the objective used.
Pupil of
objective
Appropriate size of
the aperture diaphragm
15
Focus on the specimen.
Focus on the specimen.
16
Adjust the field diaphragm
Adjust the field diaphragm
Turn the field diaphragm dial to adjust the field diaphragm so that
it almost circumscribes the field of view.
Field diaphragm
Circumscribe around
the field of view
17
View the specimen.
View the specimen.
Rotate the stage knob to move the target. If the target is not in focus,
use the focus knob to adjust the focus.
18
Turn off the power.
Turn off the power.
Turn off the power switch (press to the " "
position) for the microscope. (The power
LED on the front of the main body will turn off.)
Field diaphragm image
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Eyepiece field of view
2, Section 6 in the instruction manual for details)
Aperture diaphragm
image
Be sure to adjust the aperture
70 to 80
diaphragm each time you change
the objective.
100
(You can see the aperture diaphragm
image with the centering telescope.)
Field of view
Opening the field diaphragm too much
results in stray light entering the field
of view, generating flare and reducing
the image contrast.
Be sure to adjust the field diaphragm
each time you change the objective.
Field diaphragm image
Eyepiece field of view
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