Nikon LABOPHOT-POL Manual de instruções - Página 11
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Size of the condenser aperture diaphragm
Fig. 14
3)
Field diaphragm
(F diaphragm)
•
The field
diaphragm
is used for determin-
ing the
illuminated
area on the specimen
surface
in relation
to the field
of view of
the
microscope.
Generally,
it
is stopped
down
to such an extent
that
th'e circum-
ference
of
the
illuminated
area
circum-
scribes that
of the eyepiece
field of view.
[Note]
This
diaphragm
does not work
as
the
field
diaphragm
when
the
condenser
top
lens is swung
out
of the
optical
path.
In th is case
the
diaphragm
is recommended
to
be fully
opened
because
the
numerical
aperture
of the illumi-
nator
will
be cut
off
when
this
diaphragm
is excessively
stopped
down.
4)
Circular graduated
stage
•
The rotation
angle of the stage is readable
with the accuracy
of 0.10 via a vernier scale.
•
The stage can be clamped
at any position
using
the
stage rotation
clamp
screw
on
the vernier.
•
Remove
the
eyepiece
from
the eyepiece
tube,
adjust
the
size of
the
diaphragm,
observing
the
image
of
the
diaphragm
which
is visible
on the bright
circle of exit
pupil of objective
inside.
•
When swinging
out the top lens of the con-
denser
(for microscopy
using
4x
or lower
objective),
fully
open
the
condenser
apertu re d iaph ragm.
(2)
Conoscopic
microscopy
•
In conoscopic
microscopy,
the condenser
aperture
diaphragm
works
as a field
dia-
phragm
on the conoscopic
image surface.
Stop
down
the
diaphragm
to
such
an
extent
that
it circumscribes
the circumfer-
ence of the field of view of the conoscopic
image (exit
pupil
of objective)
to shut out
the stray light.
5)
Objectives
•
The CF Achromat
P objectives
(Strain-free)
and
CF eyepieces
adopted
in the
Nikon
POLARIZING
MICROSCOPE
LABO-
PHOT-POL
are designed
on the basis of a
concept
"Chromatic
Aberration
Free".
Exit pupil
of objective
Aperture
diaphragm
Fine focus
knob
,-Coarse
focus
knob
Fig.15
2. Manipulation of EachEleme~t } _
;1
1)
Focusing
•
The relation
between
the direction
of rota-
tion of the focus knobs and that of vertical
movement
of the stage is as indicated
in
Fig. 14.
•
One rotation
of the fine focus knob moves
the stage 0.2mm.
The
graduation
on
this
focus
knob
~
divided
into
211m.
One
rotation
of
the. coarse
focus
knob
moves the stage 4.7mm.
•
Tightness
of
the
coarse-fine
focus
knob
having
been
properly
adjusted
by
the
manufactu
rer, it should never be readjusted
in this
model
microscope
by turning
the
one knob while holding
the other.
2) Condenser
aperture
diaphragm
(A dia-
phragm)-
(1)
Orthoscopic
microscopy
•
The
condenser
aperture
diaphragm
is
provided
for
adjusting
the
numerical
aperture
(N.A)
of the illuminating
system
of microscope.
In general, when
it is stopped
down
to 70
~
80% of
the
numerical
aperture
of the
objective,
a good
image
of
appropriate
contrast
will be obtained.
(Fig. 15)
11