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After the completion of acquisition, click [x] in the windows

for real time analysis (preview) and graph to close the

windows.

After the power of the activation laser is adjusted, clicking

[Auto LP] in the [N-STORM] control window automatically

adjusts the laser power so that the number of bright points

is maintained. Also, the upper limit on the power for

automatic adjustment(%) can be specified on the [Max]

box.

2.2.2

Acquiring Images in Continuous Mode

In the example below, the following probe is used.

• Alexa647 dye (Alexa647 activated by Alexa405)

Calibration for 3D-STORM

Before the first 3D-STORM image is acquired, it is necessary to perform calibration. See "2.5 Calibration for

3D-STORM."

Click [Settings] in the [N-STORM] control window and

then select [Continuous Mode].

After making this setting, click [OK].

Select [3D-STORM] from the list at the top left of the

[N-STORM] control window.



To acquire a 2D-STORM image, select

[2D-STORM].

Chapter 2

STORM Microscopy Operation