Nikon N-STORM Manual de operação - Página 45
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3.3.3
Channel Tab
A channel tab is used to change the display of multiple channels included in a dataset. Right-clicking a channel tab allows
you to set the property of the relevant channel. It is also possible to select whether to display the [Non Specific Activation]
channel and the [Z Rejected] channel.
1
No.
1
[All]
2
Fluorescent-probe-specific
channel tab
3
[Non Specific Activation]
4
[Z Rejected]
Chapter 3
The Screens of the N-STORM Software
2
Name
Displays all the molecules for every channel. If the tabs of the
[Non Specific Activation] and [Z Rejected] channels are
displayed, the molecules in these channels are also included in
the [All] tab.
If an image is acquired on multiple channels, the
channel-specific tab is displayed.
In a cycle, molecules that are detected not in the first imaging
frame but in the second or subsequent imaging frame are
classified in a non-specific activation (NSA) channel.
Information on non-specific activation channels is used for
crosstalk subtraction.
As a result of 3D-STORM analysis, molecules that are plotted
outside the calibration range in the Z-axis direction, and
molecules whose ratio of X- and Y-axis direction image widths
exceeds the Max Axial Ratio of the Identification Settings are
classified in the Z Rejected channel. (For 3D-STORM only)
3
4
Description
41