Nikon Optiphot Manual de referência rápida - Página 6

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6. Controlling epi-fluorescence

6.1. Select filters for specific wavelength bands ("colors") by the black slider located on the

front of the microscope betwee the eyepieces and objective lens turret;

6.2. There is only room for 3 filters cubes

6.2.1. A 4

cube requires manually removing filters, usually the Cy5 and DAPI cubes

are swapped, see DMC staff for instruction on changing the 4

6.3. Xenon lamp intensity is controlled by pushing ND filters in or out of the light path

6.3.1. located behind the trinocular head (Figure 3);

6.4. Xenon excitation is controlled by a slider located between the ND filter and the xenon

lamphousing (Figure 3)

6.5. The excitation light is also blocked when the filter slider is in the "DIA" position for

transmitted light (diascopic illumination)

Figure 3. Trinocular Head and Fluorescence ND Filters

July 29, 2016

Digital Microsopy Center, University of Washington

filter

Fluorescence

Shutter

ND Filters

Trinocular

beamsplitter