Etaluma LS400 Manual de arranque - Página 4

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3. Verify that the Lumaview 500/400 version is the latest version listed in Appendix A. The version

number can be found by clicking on the Help dropdown menu and selecting About.

G. Objectives

1. The LS500 is shipped with the installed objective fully retracted. When used for the first time, it is

necessary to turn the focus knob a number of turns to reach the sample focal plane. Turning the focus

knob clockwise raises the objective and counter-clockwise lowers the objective. The safest procedure

for focusing is to raise the objective until it almost touches the sample and then lower the objective

until focus is achieved.

2. To change objectives, use the supplied 2 mm hex wrench to remove the 4 screws holding the back

panel in place. Move the panel away and with one hand reaching through the deck hole and the other

reaching from the back, carefully switch out the objective. Do not overtighten. Carry out the

replacement quickly to minimize the chance of dust falling onto the mirrored dichroic filter. If any dust

is observed on the filter, carefully blow it off using clean air such as from a compressed air can, making

sure to hold the can upright and bending the straw to aim the air directly toward the filter. If the can is

tilted, liquid can be emitted, which will require further cleaning. Other methods to remove dust may

damage the dichroic filter and must NOT be used.

3. For Meiji Plan Achromat and Plan Semi Apochromat objectives, the optional Objective Removal Tool

can also be used to change objectives. To use the Objective Removal Tool, lower it (grip end up)

through the center hole on the LS500 deck and match the inner notches of the Tool with the notched

pattern on the installed Meiji objective body. Unscrew the objective, and then lower and rotate the

Removal Tool just slightly so the notches no longer line up. This will allow lifting of the objective out of

the LS500 with the Tool. Reverse steps to install the replacement objective.

H. Power

1. For almost all imaging applications, power through the USB cable to the LS500 will be sufficient. If high

levels of illumination are required, e.g., with very dim samples, connect the electrical plug of the

included external power supply/cord into an electrical outlet and the small round plug into the round

port on the back of the LS500 (see Fig. 1).

The external power supply/cord with country-specific plug LS500 is for use with fluorescence and thus

the LS400 does not come with this power supply/cord.

I. Live Cell Imaging with LS500 in an Incubator

1. Prior to imaging in an incubator, place the LS500 inside for 12 hr to allow equilibration to the incubator

temperature.

2. Place the sample on the LS500, focus, and close the incubator door. After 60 minutes, check the focus.

If satisfactory, imaging can be initiated.

3. When live cell imaging at magnifications of 40x and above, the focus can sink several microns as the

mechanism relaxes. If the image has drifted out of focus, estimate the depth of focus change required

to bring the image back into focus and overcompensate the same amount past the point of focus.

Check the focus again after 60 minutes. The image should be in focus and imaging can be initiated.

(Repeat process as needed).

Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com

Lumascope 500/400 Startup Guide