Beckman Coulter Cytomics FC 500 Руководство пользователя - Страница 2
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15. Select the appropriate Flow Rate from the drop-down menu (recommended: Low
or Medium).
Changing Axis Name
Purpose: It may be desirable to change the word(s) on an axis. The steps below
describe how to input the axis name so that it shows on the graphs and gets stored in the
LMD file.
Things to know:
-The "Parameters" section of the Cytometer Controls window is where you can give a
name to the Detector Name term. This may guide you when adjusting voltage settings
and viewing the Parameters in the Cytometer Controls window, but the name does not get
stored anywhere that will show up on plots in CXP, FlowJo, etc.
-You cannot change the $Parameter term, which makes up the first part of the axis name
in the LMD file.
-Under FCS Header Attributes is where you can change the name of the $Stain term.
This makes up the second part of the axis name when the file is opened in FlowJo
(FCS2.0 format).
If you type "FITC" in the $Stain box for FL1, the name will look something like
this in FlowJo: FL1:: FITC
-By default, plots in CXP software use the $Stain term as the axis labels. If you would
rather have it show the $Parameter or another term, this can be adjusted in Labeling tab
of the Format Plot window (right-click on a plot and select Format Plot)
*If you are unsure which detector is being displayed on a given plot, click on the plot and
hover the mouse over the axis name until ">>>" is displayed. Click.
*In the window that opens, click on the Labeling tab.
*Find the appropriate axis (X-axis or Y-axis) and select $Parameter from the dropdown
list.
*Click OK. Now the axis should be labeled with the detector number (FL1, FL2, etc.)
*When you know which detector you would like to associate with a certain name
(perhaps the fluorescent molecule name, the antigen which the fluorescence in that
detector represents, etc.), click File.
*Click Edit FCS Header Attributes.
*In the window that opens, in the Parameters section locate the detector which you
would like to associate with a name. Click on that detector's name (i.e. FL1 log, FL1 lin,
FL2 log, FL3 log, etc.).
*On the right side of the window locate Name and click in the textbox below it.
*Type in the name you wish to show on the axis.
*Click OK.
*To make $Stain display as the axis name in a plot, open the "Format Plot" window
a. Right-click inside a plot and click Format Plot